In order to detect thermotolerant Campylobacter spp., 241 samples of fresh chicken meat, at retail in Croatia, were analysed according to a standard method, followed by biochemical test and molecular polymerase chain reaction/restriction enzyme analysis for exact species determination. Campylobacter spp. prevalence was 73.86%. Campylobacter jejuni and Campylobacter coli were isolated from 53.53 and 15.35% of the samples, respectively. In 4.98% of isolates thermotolerant Campylobacter spp. were not determined. The multilocus sequence typing method was used to evaluate genetic diversity of eight Campylobacter jejuni and four Campylobacter coli isolates. To our knowledge, these results of genotyping provided the first data on the presence of sequence types (STs) and clonal complexes (CCs) of Campylobacter jejuni and C. coli isolates in Croatia. By applying the multilocus sequence typing, a new allele of tkt gene locus was discovered and marked tkt508. The C. jejuni ST 6182 and C. coli ST 6183 genotypes were described for the first time, and all other identified genotypes were clustered in the previously described sequence types and clonal complexes. These findings provide useful information on the prevalence and epidemiology of Campylobacter jejuni and C. coli in Croatia.

中文翻译：

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耐热弯曲杆菌属的流行率。克罗地亚鸡肉中的细菌和空肠弯曲杆菌和大肠杆菌分离株小亚群的多位点序列分型。


为了检测耐热弯曲杆菌属，根据标准方法对克罗地亚零售的 241 份新鲜鸡肉样品进行了分析，然后进行生化测试和分子聚合酶链反应/限制性酶分析，以准确确定物种。弯曲杆菌属患病率为73.86%。分别从 53.53% 和 15.35% 的样品中分离出空肠弯曲菌和大肠杆菌。在 4.98% 的分离株中存在耐热弯曲杆菌属。尚未确定。采用多位点序列分型方法评估了八个空肠弯曲杆菌和四个大肠杆​​菌分离株的遗传多样性。据我们所知，这些基因分型结果提供了有关空肠弯曲杆菌和弯曲杆菌序列类型 (ST) 和克隆复合物 (CC) 存在的第一个数据。克罗地亚分离出的大肠杆菌。通过应用多位点序列分型，发现了tkt基因位点的一个新等位基因，并将其标记为tkt 508 。空肠ST 6182 和C .首次描述了大肠杆菌ST 6183 基因型，所有其他已识别的基因型都聚集在先前描述的序列类型和克隆复合体中。这些发现提供了有关克罗地亚空肠弯曲杆菌和大肠杆菌的流行率和流行病学的有用信息。