Domesticated lettuce varieties encompass much morphological variation across a range of crop type groups, with large collections of cultivars and landrace accessions maintained in genebanks. Additional variation not captured during domestication, present in ancestral wild relatives, represents a potentially rich source of alleles that can deliver to sustainable crop production. However, these large collections are difficult and costly to screen for many agronomically important traits. In this paper, we describe the generation of a diversity collection of 96 lettuce and wild species accessions that are amenable to routine phenotypic analysis and their genotypic characterization with a panel of 682 newly developed expressed sequence tag (EST)-linked KASP™ single nucleotide polymorphism (SNP) markers that are anchored to the draft Lactuca sativa genome assembly. To exemplify the utility of these resources, we screened the collection for putative sources of resistance to currant-lettuce aphid (Nasonovia ribisnigri) and carried out association analyses to look for potential SNPs linked to resistance.

中文翻译：

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寻找对抗醋栗生菜蚜虫（Nasonovia ribisnigri）的新来源。


驯化的生菜品种涵盖了一系列作物类型组的许多形态变异，基因库中保存着大量的品种和地方品种。驯化过程中未捕获的其他变异存在于祖先野生近缘种中，代表了可实现可持续作物生产的等位基因的潜在丰富来源。然而，从这些大量的收集物中筛选许多重要的农艺性状非常困难且成本高昂。在本文中，我们描述了 96 种生菜和野生物种种质的多样性集合的产生，这些种质可通过 682 个新开发的表达序列标签 (EST) 连接的 KASP™ 单核苷酸多态性进行常规表型分析及其基因型表征。 (SNP) 标记锚定到苜蓿基因组组装草案。为了举例说明这些资源的效用，我们筛选了这些资源，以寻找可能的醋栗生菜蚜虫 (Nasonovia ribisnigri) 抗性来源，并进行关联分析以寻找与抗性相关的潜在 SNP。