In recent years, exciting developments in instrument technology and experimental methodology have advanced the field of magic-angle spinning (MAS) nuclear magnetic resonance (NMR) to new heights. Contemporary MAS NMR yields atomic-level insights into structure and dynamics of an astounding range of biological systems, many of which cannot be studied by other methods. With the advent of fast MAS, proton detection, and novel pulse sequences, large supramolecular assemblies, such as cytoskeletal proteins and intact viruses, are now accessible for detailed analysis. In this review, we will discuss the current MAS NMR methodologies that enable characterization of complex biomolecular systems and will present examples of applications to several classes of assemblies comprising bacterial and mammalian cytoskeleton as well as human immunodeficiency virus 1 and bacteriophage viruses. The body of work reviewed herein is representative of the recent advancements in the field, with respect to the complexity of the systems studied, the quality of the data, and the significance to the biology.

中文翻译：

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通过魔角旋转核磁共振波谱研究超分子组装体的结构生物学


近年来，仪器技术和实验方法的令人兴奋的发展将魔角旋转（MAS）核磁共振（NMR）领域推向了新的高度。当代 MAS NMR 可以对一系列令人震惊的生物系统的结构和动力学产生原子级的见解，其中许多系统无法通过其他方法进行研究。随着快速 MAS、质子检测和新型脉冲序列的出现，大型超分子组装体（例如细胞骨架蛋白和完整病毒）现在可以进行详细分析。在这篇综述中，我们将讨论当前的 MAS NMR 方法，该方法能够表征复杂的生物分子系统，并将展示在包括细菌和哺乳动物细胞骨架以及人类免疫缺陷病毒 1 和噬菌体病毒在内的几类组件中的应用示例。本文综述的工作主体代表了该领域在所研究系统的复杂性、数据质量以及对生物学的意义方面的最新进展。