Comparative analysis of methanogenic archaea compositions and dynamics in 11 laboratory-scale continuous stirred tank reactors fed with different agricultural materials (chicken manure, cattle manure, maize straw, maize silage, distillers grains, and Jatropha press cake) was carried out by analysis of the methyl coenzyme-M reductase -subunit (mcrA) gene. Various taxa within Methanomicrobiales, Methanobacteriaceae, Methanosarcinaceae, Methanosaetaceae, and Methanomassiliicoccales were detected in the biogas reactors but in different proportions depending on the substrate type utilized as well as various process parameters. Improved coverage and higher taxonomic resolution of methanogens were obtained compared to a previous 16S rRNA gene based study of the same reactors. Some members of the genus Methanoculleus positively correlated with the relative methane content, whereas opposite correlations were found for Methanobacterium. Specific biogas production was found to be significantly correlating with Methanosarcinaceae. Statistical analysis also disclosed that some members of the genus Methanoculleus positively correlated with the ammonia level, whereas the prevalence of Methanocorpusculum, Methanobacterium, and Methanosaeta was negatively correlated with this parameter. These results suggest that the application of methanogenic archaea adapted to specific feedstock might enhance the anaerobic digestion of such waste materials in full-scale biogas reactors.

中文翻译：

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不同实验室规模厌氧消化池产甲烷菌群落的比较分析。

通过对11种实验室规模的连续搅拌釜反应器中甲烷化古生菌成分和动力学的比较分析，这些反应器采用了不同的农业原料（鸡粪，牛粪，玉米秸秆，玉米青贮饲料，酒糟和麻风树压滤饼）。甲基辅酶-M还原酶亚基（MCRA）基因。在沼气反应器中检测到了甲烷微粒菌，甲烷菌科，甲烷菌科，甲烷藻科和甲烷菌科中的各种分类群，但是根据所使用的底物类型以及各种工艺参数，其比例不同。与以前的基于16S rRNA基因的相同反应器研究相比，产甲烷菌具有更高的覆盖范围和更高的分类学分辨率。属的一些成员Methanoculleus与相对甲烷含量正相关，而被发现的相对相关性甲烷。发现特定的沼气产量与甲烷菌科显着相关。统计分析还显示，甲烷菌属的某些成员与氨水平呈正相关，而盛行Methanocorpusculum，甲烷，和Methanosaeta呈负该参数相关。这些结果表明，适用于特定原料的产甲烷古菌的应用可能会增强沼气反应器中此类废料的厌氧消化。