Solid-stemmed spring wheat cultivars (Triticum aestivum L.) are resistant to the stem sawfly (Cephus cinctus Nort.) and lodging. Anthers of 24 spring wheat cultivars with varying content of pith in the stem were used in the experiment. All were classified into three groups: solid, medium-solid and hollow stems. There was considerable influence of the cultivar on callus formation and green plant regeneration. The highest efficiency of green plant regeneration (24%) was observed for the solid-stemmed AC Abbey cultivar. There was no regeneration from the explants of four cultivars: CLTR 7027, Alentejano, Marquis and Bombona. Principal component analysis showed no differences between the cases under observation (callus induction and green plant regeneration) in their response to pre-treatment temperatures (4 and 8°C). The examination of the effects of various auxin types in the induction medium on callus formation and green plant regeneration revealed that the strongest stimulation of these processes was observed in the C17 medium with 2,4-D and dicamba. The efficiency of callus formation and green plant regeneration was greater in solid-stemmed cultivars than in hollow-stemmed cultivars.

中文翻译：

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在花药培养中，固茎春小麦品种比空心茎品种具有更好的雄激素响应。

固态梗春小麦品种（Triticum aestivum L.）对茎锯蝇（Cephus cinctus Nort。）和倒伏都有抗性。实验中使用了24个春小麦品种的花药，其中茎中的髓含量变化不一。全部分为三类：实心，中实心和空心茎。该品种对愈伤组织形成和绿色植物再生有相当大的影响。对于固态梗的AC Abbey品种，观察到最高的绿色植物再生效率（24％）。四个品种的外植体没有再生：CLTR 7027，Alentejano，Marquis和Bombona。主成分分析表明，观察到的案例（愈伤组织诱导和绿色植物再生）对预处理温度（4和8°C）的响应没有差异。检查诱导培养基中各种生长素类型对愈伤组织形成和绿色植物再生的影响，结果表明，在含有2,4-D和麦草畏的C17培养基中观察到了这些过程的最强刺激。实心栽培品种的愈伤组织形成和绿色植物再生的效率高于空心栽培品种。