Obtaining oat DH lines is only effective via wide crossing with maize. Seven hundred haploid embryos from 21 single F1 progeny obtained from wide crosses with maize were isolated, divided into four groups according to their size (<0.5 mm, 0.5-0.9 mm, 1.0-1.4 mm, and ≥1.5 mm), and transferred into 190-2 regeneration medium with different growth regulators: 0.5 mg L-1 kinetin (KIN) and 0.5 mg L-1 1-naphthaleneacetic acid (NAA); 1 mg L-1 zeatin (ZEA) and 0.5 mg L-1 NAA; or 1 mg L-1 dicamba (DIC), 1 mg L-1 picloram (PIC), and 0.5 mg L-1 kinetin (KIN). Among all isolated embryos, approximately 46.1% were between 1.0-1.4 mm, while the smallest group of embryos (7.1%) were those <0.5 mm. The ability of haploid embryos to germinate varied depending on oat genotypes and the size of embryos. Haploid embryos <0.5 mm were globular and did not germinate, whereas embryos ≥1.5 mm had clearly visible coleoptiles, radicles, and scutella, and were able to germinate. Germination of oat haploid embryos varied depending on growth regulators in the regeneration medium. Most haploid embryos germinated on medium with 0.5 mg L-1 NAA and 0.5 mg L-1 KIN, while the fewest germinated on medium with 1 mg L-1 DIC, 1 mg L-1 PIC, and 0.5 mg L-1 KIN. One hundred thirty germinated haploid embryos converted into haploid plants. Fifty oat DH lines were obtained after colchicine treatment.

中文翻译：

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燕麦（Avena sativa L.）单倍体胚胎向植物的转化与胚胎的发育阶段和再生培养基有关。

获得燕麦DH系仅通过与玉米广泛杂交才有效。从与玉米的宽杂交获得的21个单F1子代中分离出700个单倍体胚胎，并根据它们的大小（<0.5 mm，0.5-0.9 mm，1.0-1.4 mm和≥1.5mm）分为四组，并转移到具有不同生长调节剂的190-2再生培养基：0.5 mg L-1激动素（KIN）和0.5 mg L-1 1-萘乙酸（NAA）；1毫克L-1玉米素（ZEA）和0.5毫克L-1 NAA；或1毫克L-1麦草畏（DIC），1毫克L-1吡咯烷（PIC）和0.5毫克L-1激动素（KIN）。在所有分离的胚胎中，约46.1％的胚胎在1.0-1.4毫米之间，而最小的一组胚胎（7.1％）是那些<0.5毫米的胚胎。单倍体胚发芽的能力根据燕麦的基因型和胚的大小而变化。单倍体胚胎<0。5毫米为球形，不发芽，而≥1.5毫米的胚具有清晰可见的胚芽鞘，胚根和黄cut，并且能够发芽。燕麦单倍体胚的发芽取决于再生培养基中的生长调节剂。大多数单倍体胚胎在含有0.5 mg L-1 NAA和0.5 mg L-1 KIN的培养基上发芽，而最少的则在含有1 mg L-1 DIC，1 mg L-1 PIC和0.5 mg L-1 KIN的培养基上发芽。一百三十个发芽的单倍体胚胎转化为单倍体植物。秋水仙碱处理后获得五十个燕麦DH系。而在含有1 mg L-1 DIC，1 mg L-1 PIC和0.5 mg L-1 KIN的培养基上发芽的最少。一百三十个发芽的单倍体胚胎转化为单倍体植物。秋水仙碱处理后获得五十个燕麦DH系。而在含有1 mg L-1 DIC，1 mg L-1 PIC和0.5 mg L-1 KIN的培养基上发芽的最少。一百三十个发芽的单倍体胚胎转化为单倍体植物。秋水仙碱处理后获得五十个燕麦DH系。