Lymphocyte subpopulations producing cytokines and exerting regulatory functions represent key immune elements. Given their reciprocal interdependency lymphocyte subpopulations are usually assayed as diagnostic panels, rather than single biomarkers for specialist clinical use. This retrospective analysis on lymphocyte subpopulations, analyzed over the last few years in an outpatient laboratory in Northeast Italy, contributes to the establishment of reference values for several regulatory lymphocytes currently lacking such reference ranges for the general population. Mean values and ranges in a sample of Caucasian patients (mean age 42±8,5 years), were provided for Th1, Th2, Th17, Th-reg, Tc-reg, Tc-CD57+ and B1 lymphocytes. The results are consistent with what is found in literature for the single subtypes and are: Th1 157.8±60.3/µl (7.3%±2.9); Th2 118.2±52.2/µl (5.4%±2.5); Th17 221.6±90.2/µl (10.5%±4.4); Th-reg 15.1±10.2/µl (0.7%±0.4); Tc-reg 5.8±4.7/µl (0.3%±0.2); Tc-CD57+ 103.7±114.1/µl (4.6%±4.7); B1 33.7±22.8/µl (1.5%±0.9); (Values are mean±SD). The results show that despite their variability, mean values are rather consistent in all age or sex groups and can be used as laboratory internal reference for this regulatory panel. Adding regulatory cells to lymphocyte subpopulations panels allows a more complete view of the state of the subject's immune network balance, thus improving the personalization and the “actionability” of diagnostic data in a systems medicine perspective.

中文翻译：

---

一组细胞因子和调节性淋巴细胞亚群的参考值

产生细胞因子和发挥调节功能的淋巴细胞亚群代表了关键的免疫元件。鉴于它们相互依赖的淋巴细胞亚群通常作为诊断面板进行检测，而不是专科临床使用的单一生物标志物。过去几年在意大利东北部的门诊实验室进行的淋巴细胞亚群回顾性分析有助于为目前缺乏针对一般人群的此类参考范围的几种调节性淋巴细胞建立参考值。提供了白人患者样本（平均年龄 42±8.5 岁）中 Th1、Th2、Th17、Th-reg、Tc-reg、Tc-CD57+ 和 B1 淋巴细胞的平均值和范围。结果与文献中单一亚型的结果一致，为：Th1 157.8±60.3/μl (7.3%±2.9)；Th2 118.2±52.2/μl (5.4%±2.5)；Th17 221.6±90.2/μl (10.5%±4.4)；Th-reg 15.1±10.2/µl (0.7%±0.4); Tc-reg 5.8±4.7/µl (0.3%±0.2); Tc-CD57+ 103.7±114.1/μl (4.6%±4.7)；B1 33.7±22.8/μl (1.5%±0.9)；（数值是平均值±SD）。结果表明，尽管存在差异，但所有年龄或性别组的平均值都相当一致，可用作该监管小组的实验室内部参考。将调节细胞添加到淋巴细胞亚群面板可以更完整地了解受试者的免疫网络平衡状态，从而从系统医学的角度提高诊断数据的个性化和“可操作性”。结果表明，尽管存在差异，但所有年龄或性别组的平均值都相当一致，可用作该监管小组的实验室内部参考。将调节细胞添加到淋巴细胞亚群面板可以更完整地了解受试者的免疫网络平衡状态，从而从系统医学的角度提高诊断数据的个性化和“可操作性”。结果表明，尽管存在差异，但所有年龄或性别组的平均值都相当一致，可用作该监管小组的实验室内部参考。将调节细胞添加到淋巴细胞亚群面板可以更完整地了解受试者的免疫网络平衡状态，从而从系统医学的角度提高诊断数据的个性化和“可操作性”。