Immune cells (leukocytes or white blood cells) move actively and sensitively based on body conditions. Despite their important role as protectors inside the body, it is difficult to directly observe the spatiotemporal momentum of leukocytes. With advances in imaging technology, the introduction of two-photon microscopy has enabled researchers to look deeper inside tissues in a three-dimensional manner. In observations of immune cell movement along the blood vessel, vascular permeability and innate immune cell movements remain unclear. Here, we describe the neutrophil extravasation cascade, which were observed using a two-photon intravital imaging technique. We also provide evidence for novel mechanisms such as neutrophil body extension and microparticle formation as well as their biological roles during migration.

中文翻译：

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中性粒细胞外渗级联：我们可以从双光子活体成像中学到什么？

免疫细胞（白细胞或白细胞）根据身体状况积极而敏感地移动。尽管它们在体内具有重要的保护作用，但很难直接观察白细胞的时空动量。随着成像技术的进步，双光子显微镜的引入使研究人员能够以三维方式更深入地观察组织内部。在观察沿血管的免疫细胞运动时，血管通透性和先天免疫细胞运动仍不清楚。在这里，我们描述了使用双光子活体成像技术观察到的中性粒细胞外渗级联。我们还提供了新机制的证据，例如中性粒细胞体扩展和微粒形成以及它们在迁移过程中的生物学作用。