Diabetes constitutes a worldwide epidemic that affects all ethnic groups. Cell therapy is one of the best alternatives of treatment, by providing an effective way to regenerate insulin-producing cells lost during the course of the disease, but many issues remain to be solved. Several groups have been working in the development of a protocol capable of differentiating Mesenchymal Stem Cells (MSCs) into physiologically sound Insulin Producing Cells (IPCs). In order to obtain a simple, fast and direct method, we propose in this manuscript the induction of MSCs to express NESTIN in a short time period (2 h), proceeded by incubation in a low glucose induced medium (24 h) and lastly by incubation in a high glucose medium. Samples from cell cultures incubated in high glucose medium from 12 to 168 h were obtained to detect the expression of INSULIN-1, INSULIN -2, PDX-1 and GLUT-2 genes. Induced cells were exposed to a glucose challenge, in order to assess the production of insulin. This method allowed us to obtain cells expressing PDX-1, which resembles a progenitor insulin-producing cell.

中文翻译：

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诱导Nestin早期表达是间充质干细胞的标志性标志物PDX-1的表达是其转化为胰岛素生产细胞的易患因素。

糖尿病是影响所有种族的世界性流行病。通过提供一种有效的方法来再生在疾病过程中丢失的胰岛素产生细胞，细胞疗法是治疗的最佳替代方法之一，但许多问题仍有待解决。几个小组一直在致力于开发一种能够将间充质干细胞（MSC）分化为生理上合理的胰岛素产生细胞（IPC）的协议。为了获得一种简单，快速，直接的方法，我们建议在本文中诱导MSC在短时间内（2 h）表达NESTIN，方法是在低葡萄糖诱导培养基中孵育（24 h），最后通过在高葡萄糖培养基中孵育。获得在高葡萄糖培养基中培养12至168小时的细胞培养物样品，以检测INSULIN-1的表达，胰岛素-2，PDX-1和GLUT-2基因。为了评估胰岛素的产生，将诱导的细胞暴露于葡萄糖激发。这种方法使我们能够获得表达PDX-1的细胞，该细胞类似于产生胰岛素的祖细胞。