Acinetobacter baumannii is a major opportunistic pathogen in healthcare settings worldwide. In Iran, there are only few reports on the prevalence of aminoglycoside resistance genes among A. baumannii isolates. The aim of this study was to investigate the existence of aminoglycoside-modifying enzyme (AME) genes from A. baumannii strains collected at a university teaching hospital in Iran. One hundred A. baumannii strains were collected between 2014 and 2015 from hospitalized patients at Loghman Hakim Hospital, Tehran, Iran. Antimicrobial susceptibility was determined by disk diffusion method according to the Clinical and Laboratory Standards Institute recommendations. The DNA was extracted using a kit obtained from Bioneer Co. (Korea) and was used as a template for polymerase chain reaction. The most active antimicrobial agent against these strains was colistin. The rate of extended-spectrum cephalosporin resistance was 97%. The aadA1, aadB, aac(6')-Ib, and aac(3)-IIa genes were found in 85%, 77%, 72%, and 68% of A. baumannii isolates, respectively. This study showed a high prevalence rate of AME genes in A. baumannii. This prevalence rate has explained that further aminoglycoside resistance genes may have role in the resistance of clinical isolates of A. baumannii. Therefore, control and treatment of serious infections caused by this opportunistic pathogen should be given more consideration.

中文翻译：

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鲍曼不动杆菌临床分离物中氨基糖苷修饰酶基因的分子检测。

鲍曼不动杆菌是全世界医疗机构中的主要机会病原体。在伊朗，关于鲍曼不动杆菌分离株中氨基糖苷抗性基因盛行的报道很少。这项研究的目的是调查存在于伊朗一所大学教学医院的鲍曼不动杆菌菌株中氨基糖苷修饰酶（AME）基因的存在。在2014年至2015年之间，从伊朗德黑兰Loghman Hakim医院的住院患者中收集了一百株鲍曼不动杆菌。根据临床和实验室标准协会的建议，通过圆盘扩散法测定了抗菌药的敏感性。使用从Bioneer Co.（韩国）获得的试剂盒提取DNA，并将其用作聚合酶链反应的模板。针对这些菌株最活跃的抗菌剂是粘菌素。广谱头孢菌素耐药率为97％。在鲍曼不动杆菌的分离物中分别发现aadA1，aadB，aac（6'）-Ib和aac（3）-IIa基因。这项研究表明鲍曼不动杆菌中AME基因的高患病率。该患病率解释了进一步的氨基糖苷抗性基因可能在鲍曼不动杆菌临床分离株的抗性中起作用。因此，应该更多地考虑控制和治疗这种机会病原体引起的严重感染。这项研究表明鲍曼不动杆菌中AME基因的高患病率。该患病率解释了进一步的氨基糖苷抗性基因可能在鲍曼不动杆菌临床分离株的抗性中起作用。因此，应该更多地考虑控制和治疗这种机会病原体引起的严重感染。这项研究表明鲍曼不动杆菌中AME基因的高患病率。该患病率解释了进一步的氨基糖苷抗性基因可能在鲍曼不动杆菌临床分离株的抗性中起作用。因此，应该更多地考虑控制和治疗这种机会病原体引起的严重感染。