The objective of this study was to conduct a survey about fungi associated with leaves from two different maize plant lineages and to analyze their microbiota diversity. Isolated fungi were identified by morphological analysis and molecular taxonomy was performed using ITS1-5.8S-ITS2 rDNA. About 27 fungi morphotypes were obtained, 15 of them were from the first maize lineage. About 86.7% of the individuals belonged to the Dothideomycetes class (Phoma sorghina, Epicocum nigrum, Cladosporium sp., Bipolaris zeicola, and Alternaria alternata complex) and 13.3% to the Sordariomycetes class (Diaporthe/Phomopsis sp. and Nigrospora sp.). This ratio was opposite in the other maize lineage with 25.0% of Dothideomycetes (E. nigrum and Pleosporales) and 75.0% of Sordariomycetes (Gibberella fujikuroi complex, Fusarium graminearum complex, Diaporthe/Phomopsis sp., and Nigrospora sp.). By concerning the analyses of morphological characteristics and molecular phylogeny, this study intended to identify the groups of saprophytic, phytopathogenic, and mycotoxin fungi, which differently co-inhabit leaf tissue of maize plants in both tested lineages.

中文翻译：

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与巴西南部农田种植的玉米叶片相关的内生真菌群落组成。

这项研究的目的是对来自两个不同玉米植物谱系的叶片相关真菌进行调查，并分析其微生物群多样性。通过形态分析鉴定分离的真菌，并使用ITS1-5.8S-ITS2 rDNA进行分子分类。获得了大约27种真菌形态型，其中15种来自第一个玉米谱系。大约86.7％的人属于直链丝菌类（高粱，黑胡椒，Cladosporium sp。，Bipolaris zeicola和Alternaria alternata复合体），而13.3％的球菌属于Sordariomycetes类（Diaporthe / Phomopsis sp。和Nigrospora sp。）。在其他玉米谱系中，该比例相反，分别为25.0％的丝状丝菌（E. nigrum和Pleosporales）和75.0％的Sordariomycetes（Gibberella fujikuroi复合物，Fusarium graminearum复合物，Diaporthe / Phomopsis sp。和Nigrospora sp。）。通过对形态特征和分子系统发育的分析，本研究旨在鉴定腐生真菌，植物致病真菌和霉菌毒素真菌，它们在两种被测谱系中均共同存在于玉米植物的叶片组织中。