Optical sectioning techniques offer the ability to acquire three‐dimensional information from various organ tissues by discriminating between the desired in‐focus and out‐of‐focus (background) signals. Alternative techniques to confocal, such as active structured illumination, exist for fast optically sectioned images, but they require individual axial planes to be imaged consecutively. In this article, an imaging technique (THIN), by utilizing active Talbot illumination in 3D and multiplexed holographic Bragg filters for depth discrimination, is demonstrated for imaging in vivo 3D biopsy without mechanical or optical axial scanning.

中文翻译：

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Talbot全息照像非扫描（THIN）荧光显微镜。

光学切片技术提供了通过区分所需的焦点对准和焦点对准（背景）信号来从各种器官组织获取三维信息的能力。共焦的替代技术（例如主动结构照明）可用于快速光学剖切的图像，但它们需要单独的轴向平面连续成像。在本文中，演示了一种成像技术（THIN），该技术通过利用3D中的主动Talbot照明和用于深度识别的多路全息全息滤镜进行成像，无需机械或光学轴向扫描即可对体内3D活检成像。