Objective

To investigate the relationship between the response to influenza vaccination and the ability to produce proinflamatory cytokines in elderly subjects.

Methods

Whole blood samples from 25 elderly subjects collected before influenza vaccination were stimulated with the influenza vaccine in order to evaluate the secretion of five specific cytokines: TNFα, IFNα, IFNγ, IL2 and IL10. The results were correlated with the increased HAI antibody titres two weeks after vaccination.

Results

Only 30% of elderly individuals seroconverted after vaccination. Although 50 to 70% of the cohort did not produce TNFα, IFNα, IFNγ, IL2 or IL10, all of the individuals who seroconverted were able to produceTNFα. Furthermore production of IFNγ, with or without production of IFNα/β, was not associated with a better response to the vaccine.

Conclusion

Production of TNFα appears to be primordial for an efficient vaccine response, and may provide a predictive marker for the humoral response to vaccination. It may also provide the basis for evaluating agents designed to rescue TNFα-producing cells. This study emphasises a need to rescue TNF-producing cell function.

中文翻译：

---

体内TNF-α的产生预示着脆弱的老年人群对流感疫苗的免疫反应。

客观的

调查对老年人接种流感疫苗的反应与产生促炎细胞因子的能力之间的关系。

方法

为了评估五种特定细胞因子（TNFα，IFNα，IFNγ，IL2和IL10）的分泌，用流感疫苗刺激了来自25名老年受试者在接种流感疫苗之前收集的全血样品。结果与疫苗接种两周后HAI抗体滴度增加有关。

结果

接种疫苗后，只有30％的老年人发生血清转化。尽管该队列中有50％到70％的人不产生TNFα，IFNα，IFNγ，IL2或IL10，但所有血清转化的个体都能够产生TNFα。此外，产生或不产生IFNα/β的IFNγ与疫苗的更好应答无关。

结论

TNFα的产生似乎对有效的疫苗反应至关重要，并且可以为疫苗的体液反应提供预测标记。它也可以为评估旨在拯救产生TNFα的细胞的试剂提供基础。这项研究强调了挽救产生TNF的细胞功能的需要。