Despite that a bacterial genome is complicated by large numbers of horizontally transferred (HT) genes and function unknown hypothetical (FUN) genes, the Genic-Transcriptional-Stop-Signals-Ratio (TSSR) of a genome shows that HT and FUN genes are complementary to all other genes in the genome. When HT or certain FUN genes are omitted from the Escherichia coli K-12 genome, its Genomic-TSSR value becomes totally incomparable to other E. coli strains. The Genic-TSSR correlation tree of a pathogen shows that some FUN genes would form a unique cluster. Removing these genes by site-specific mutation or gene-knockout should lead to the demise of this pathogen.

中文翻译：

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一种新的药物设计策略：通过使它们的假设基因失活来杀死耐药细菌。

尽管细菌基因组由于大量的水平转移（HT）基因和功能未知的假设（FUN）基因而变得复杂，但基因组的遗传-转录-停止-信号-比率（TSSR）表明HT和FUN基因是互补的基因组中的所有其他基因。当从大肠杆菌K-12基因组中缺失HT或某些FUN基因时，其基因组TSSR值就变得与其他大肠杆菌菌株完全无法比拟。病原体的Genic-TSSR相关树显示某些FUN基因将形成一个独特的簇。通过位点特异性突变或基因敲除去除这些基因应导致该病原体死亡。