Cassava (Manihot esculenta Crantz) is a tropical root crop and sensitive to low temperature. However, it is poorly to know how cassava can modify its metabolism and growth to adapt to cold stress. An investigation aimed at a better understanding of cold-tolerant mechanism of cassava plantlets was carried out with the approaches of physiology and proteomics in the present study. The principal component analysis of seven physiological characteristics showed that electrolyte leakage (EL), chlorophyll content, and malondialdehyde (MDA) may be the most important physiological indexes for determining cold-resistant abilities of cassava. The genome-wide proteomic analysis showed that 20 differential proteins had the same patterns in the apical expanded leaves of cassava SC8 and Col1046. They were mainly related to photosynthesis, carbon metabolism and energy metabolism, defense, protein synthesis, amino acid metabolism, signal transduction, structure, detoxifying and antioxidant, chaperones, and DNA-binding proteins, in which 40 % were related with photosynthesis. The remarkable variation in photosynthetic activity and expression level of peroxiredoxin is closely linked with expression levels of proteomic profiles. Moreover, analysis of differentially expressed proteins under cold stress is an important step toward further elucidation of mechanisms of cold stress resistance.

中文翻译：

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木薯幼苗对冷胁迫响应的蛋白质组学比较分析

木薯 (Manihot esculenta Crantz) 是一种热带块根作物，对低温敏感。然而，目前尚不清楚木薯如何改变其新陈代谢和生长以适应冷胁迫。本研究采用生理学和蛋白质组学的方法进行了一项旨在更好地了解木薯幼苗耐寒机制的调查。七项生理特征的主成分分析表明，电解质渗漏（EL）、叶绿素含量和丙二醛（MDA）可能是决定木薯抗寒能力的最重要生理指标。全基因组蛋白质组学分析表明，在木薯 SC8 和 Col1046 的顶端展开叶中，有 20 种差异蛋白具有相同的模式。它们主要与光合作用有关，碳代谢和能量代谢、防御、蛋白质合成、氨基酸代谢、信号转导、结构、解毒和抗氧化、分子伴侣和DNA结合蛋白，其中40%与光合作用有关。过氧化物酶的光合活性和表达水平的显着变化与蛋白质组学谱的表达水平密切相关。此外，分析冷胁迫下差异表达的蛋白质是进一步阐明冷胁迫抗性机制的重要一步。过氧化物酶的光合活性和表达水平的显着变化与蛋白质组学谱的表达水平密切相关。此外，分析冷胁迫下差异表达的蛋白质是进一步阐明冷胁迫抗性机制的重要一步。过氧化物酶的光合活性和表达水平的显着变化与蛋白质组学谱的表达水平密切相关。此外，分析冷胁迫下差异表达的蛋白质是进一步阐明冷胁迫抗性机制的重要一步。