Escherichia coli has developed sophisticated means to sense, respond, and adapt in stressed environment. It has served as a model organism for studies in molecular genetics and physiology since the 1960s. Stress response genes are induced whenever a cell needs to adapt and survive under unfavorable growth conditions. Two of the possible important genes are rpoS and bolA. The rpoS gene has been known as the alternative sigma (σ) factor, which controls the expression of a large number of genes, which are involved in responses to various stress factors as well as transition to stationary phase from exponential form of growth. Morphogene bolA response to stressed environment leads to round morphology of E. coli cells, but little is known about its involvement in biofilms and its development or maintenance. This study has been undertaken to address the adherence pattern and formation of biofilms by E. coli on stainless steel, polypropylene, and silicone surfaces after 24 h of growth at 37 °C. Scanning electron microscopy was used for direct examination of the cell attachment and biofilm formation on various surfaces and it was found that, in the presence of bolA, E. coli cells were able to attach to the stainless steel and silicone very well. By contrast, polypropylene surface was not found to be attractive for E. coli cells. This indicates that bolA responded and can play a major role in the presence and absence of rpoS in cell attachment.

中文翻译：

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bolA和rpoS基因在大肠杆菌K-12 MG1655在聚丙烯，不锈钢和有机硅表面上的生物膜形成和粘附模式中的作用。

大肠埃希氏菌已开发出先进的方法来感知，响应和适应压力大的环境。自1960年代以来，它已成为分子生物学和生理学研究的典范生物。每当细胞需要适应并在不利的生长条件下存活时，就会诱导应激反应基因。两个可能的重要基因是rpoS和bolA。rpoS基因被称为替代sigma（σ）因子，它控制大量基因的表达，这些基因参与对各种胁迫因子的响应以及从指数形式的生长向固定相的转变。Morphogene bolA对压力环境的反应导致大肠杆菌细胞呈圆形形态，但鲜为人知的是其参与生物膜及其发育或维持的过程。这项研究旨在解决大肠杆菌在37°C下生长24小时后在不锈钢，聚丙烯和硅树脂表面上的粘附模式和生物膜形成的问题。扫描电子显微镜用于直接检查各种表面上的细胞附着和生物膜形成，发现在存在bolA的情况下，大肠杆菌细胞能够很好地附着在不锈钢和硅酮上。相反，未发现聚丙烯表面对大肠杆菌细胞具有吸引力。这表明bolA可以应答，并且在细胞附着中是否存在rpoS时可以起主要作用。扫描电子显微镜用于直接检查各种表面上的细胞附着和生物膜形成，发现在存在bolA的情况下，大肠杆菌细胞能够很好地附着在不锈钢和硅酮上。相反，未发现聚丙烯表面对大肠杆菌细胞具有吸引力。这表明bolA可以应答，并且在细胞附着中是否存在rpoS时可以起主要作用。扫描电子显微镜用于直接检查各种表面上的细胞附着和生物膜形成，发现在存在bolA的情况下，大肠杆菌细胞能够很好地附着在不锈钢和硅酮上。相反，未发现聚丙烯表面对大肠杆菌细胞具有吸引力。这表明bolA可以应答，并且在细胞附着中是否存在rpoS时可以起主要作用。