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A review of modern approaches to the hydrodynamic characterisation of polydisperse macromolecular systems in biotechnology.
Biotechnology and Genetic Engineering Reviews ( IF 6.5 ) Pub Date : 2015-02-16 , DOI: 10.1080/02648725.2014.994870
Richard B Gillis 1 , Arthur J Rowe , Gary G Adams , Stephen E Harding
Affiliation  

This short review considers the range of modern techniques for the hydrodynamic characterisation of macromolecules – particularly large glycosylated systems used in the food, biopharma and healthcare industries. The range or polydispersity of molecular weights and conformations presents special challenges compared to proteins. The review is aimed, without going into any great theoretical or methodological depth, to help the Industrial Biotechnologist choose the appropriate methodology or combination of methodologies for providing the detail he/she needs for particular applications.



中文翻译:

对生物技术中多分散大分子系统流体动力学表征的现代方法的综述。

这篇简短的评论考虑了用于大分子流体动力学表征的现代技术范围,尤其是食品,生物制药和医疗保健行业中使用的大型糖基化系统。与蛋白质相比,分子量和构象的范围或多分散性提出了特殊的挑战。审查的目的是在不深入任何理论或方法论深度的情况下,帮助工业生物技术人员选择适当的方法论或方法论的组合,以提供他/她针对特定应用所需的详细信息。

更新日期:2015-02-16
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