Low molecular weight glutenin subunits (LMW-GS) encoded by the Glu-3 loci are known to contribute to wheat breadmaking quality. However, the specific effect of individual Glu-3 alleles is not well understood due to their complex protein banding patterns in SDS-PAGE and tight linkage with gliadins at the Gli-1 locus. Using DNA markers and a backcross program we developed a set of nine near isogenic lines (NILs) including different Glu-A3/GliA-1 or Glu-B3/Gli-B1 alleles in the genetic background of the Argentine variety ProINTA Imperial. The nine NILs and the control were evaluated in three different field trials in Argentina. Significant genotype-by-environment interactions were detected for most quality parameters indicating that the effects of the Glu-3/Gli-1 alleles are modulated by environmental differences. None of the NILs showed differences in total flour protein content, but relative changes in the abundance of particular classes of proteins cannot be ruled out. On average, the Glu-A3f, Glu-B3b, Glu-B3g and Glu-B3iMan alleles were associated with the highest values in gluten strength-related parameters, while Glu-A3e, Glu-B3a and Glu-B3iChu were consistently associated with weak gluten and low quality values. The value of different Glu3/Gli-1 allele combinations to improve breadmaking quality is discussed.

中文翻译：

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Glu-3/Gli-1 基因座等位基因变异对六倍体小麦 (Triticum aestivum L.) 面包制作质量参数的影响

已知由 Glu-3 基因座编码的低分子量麦谷蛋白亚基 (LMW-GS) 有助于提高小麦面包制作质量。然而，由于它们在 SDS-PAGE 中的复杂蛋白质条带模式以及与 Gli-1 位点醇溶蛋白的紧密联系，单个 Glu-3 等位基因的具体作用尚不清楚。使用 DNA 标记和回交程序，我们在阿根廷品种 ProINTA Imperial 的遗传背景中开发了一组九个近等基因系 (NIL)，包括不同的 Glu-A3/GliA-1 或 Glu-B3/Gli-B1 等位基因。九个 NIL 和对照在阿根廷的三个不同的田间试验中进行了评估。大多数质量参数检测到显着的基因型与环境的相互作用，表明 Glu-3/Gli-1 等位基因的作用受环境差异的调节。NIL 均未显示总面粉蛋白质含量存在差异，但不能排除特定类别蛋白质丰度的相对变化。平均而言，Glu-A3f、Glu-B3b、Glu-B3g 和 Glu-B3iMan 等位基因与面筋强度相关参数的最高值相关，而 Glu-A3e、Glu-B3a 和 Glu-B3iChu 始终与弱相关面筋和低质量值。讨论了不同 Glu3/Gli-1 等位基因组合对提高面包制作质量的价值。Glu-B3a 和 Glu-B3iChu 始终与弱面筋和低质量值相关。讨论了不同 Glu3/Gli-1 等位基因组合对提高面包制作质量的价值。Glu-B3a 和 Glu-B3iChu 始终与弱面筋和低质量值相关。讨论了不同 Glu3/Gli-1 等位基因组合对提高面包制作质量的价值。