当前位置: X-MOL 学术Microbiol. Mol. Biol. Rev. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Regulatory RNAs in Bacillus subtilis: a Gram-Positive Perspective on Bacterial RNA-Mediated Regulation of Gene Expression.
Microbiology and Molecular Biology Reviews ( IF 8.0 ) Pub Date : 2016-10-28 , DOI: 10.1128/mmbr.00026-16
Ruben A T Mars 1 , Pierre Nicolas 2 , Emma L Denham 1 , Jan Maarten van Dijl 3
Affiliation  

Bacteria can employ widely diverse RNA molecules to regulate their gene expression. Such molecules include trans-acting small regulatory RNAs, antisense RNAs, and a variety of transcriptional attenuation mechanisms in the 5' untranslated region. Thus far, most regulatory RNA research has focused on Gram-negative bacteria, such as Escherichia coli and Salmonella. Hence, there is uncertainty about whether the resulting insights can be extrapolated directly to other bacteria, such as the Gram-positive soil bacterium Bacillus subtilis. A recent study identified 1,583 putative regulatory RNAs in B. subtilis, whose expression was assessed across 104 conditions. Here, we review the current understanding of RNA-based regulation in B. subtilis, and we categorize the newly identified putative regulatory RNAs on the basis of their conservation in other bacilli and the stability of their predicted secondary structures. Our present evaluation of the publicly available data indicates that RNA-mediated gene regulation in B. subtilis mostly involves elements at the 5' ends of mRNA molecules. These can include 5' secondary structure elements and metabolite-, tRNA-, or protein-binding sites. Importantly, sense-independent segments are identified as the most conserved and structured potential regulatory RNAs in B. subtilis. Altogether, the present survey provides many leads for the identification of new regulatory RNA functions in B. subtilis.

中文翻译:

枯草芽孢杆菌中的调控RNA:细菌RNA介导的基因表达调控的革兰氏阳性视角。

细菌可以使用多种RNA分子来调节其基因表达。这样的分子包括反式作用的小调节RNA,反义RNA,以及在5'非翻译区的各种转录衰减机制。迄今为止,大多数监管RNA研究都集中在革兰氏阴性细菌,例如大肠杆菌和沙门氏菌。因此,对于所得的见解是否可以直接推断到其他细菌(如革兰氏阳性土壤细菌枯草芽孢杆菌)存在不确定性。最近的一项研究在枯草芽孢杆菌中鉴定了1,583个假定的调节性RNA,在104种条件下对其表达进行了评估。在这里,我们回顾了目前对枯草芽孢杆菌中基于RNA的调控的了解,我们根据新发现的推测性调控RNA在其他细菌中的保守性以及它们预测的二级结构的稳定性,对它们进行了分类。我们目前对公开数据的评估表明,枯草芽孢杆菌中RNA介导的基因调控主要涉及mRNA分子5'端的元件。这些可以包括5'二级结构元件和代谢物,tRNA或蛋白质结合位点。重要的是,与感觉无关的片段被鉴定为枯草芽孢杆菌中最保守和结构化的潜在调控RNA。总之,本调查为枯草芽孢杆菌中新的调控RNA功能的鉴定提供了许多线索。我们目前对公开数据的评估表明,枯草芽孢杆菌中RNA介导的基因调控主要涉及mRNA分子5'端的元件。这些可以包括5'二级结构元件和代谢物,tRNA或蛋白质结合位点。重要的是,与感觉无关的片段被鉴定为枯草芽孢杆菌中最保守和结构化的潜在调控RNA。总之,本调查为枯草芽孢杆菌中新的调控RNA功能的鉴定提供了许多线索。我们目前对公开数据的评估表明,枯草芽孢杆菌中RNA介导的基因调控主要涉及mRNA分子5'端的元件。这些可以包括5'二级结构元件和代谢物,tRNA或蛋白质结合位点。重要的是,与感觉无关的片段被鉴定为枯草芽孢杆菌中最保守和结构化的潜在调控RNA。总之,本调查为枯草芽孢杆菌中新的调控RNA功能的鉴定提供了许多线索。枯草杆菌。总之,本调查为枯草芽孢杆菌中新的调控RNA功能的鉴定提供了许多线索。枯草杆菌。总之,本调查为枯草芽孢杆菌中新的调控RNA功能的鉴定提供了许多线索。
更新日期:2019-11-01
down
wechat
bug