BACKGROUND The acquisition of iron is important for the pathogenicity of bacteria and blood. Three different culture environments (Fe stimulation, blood agar plate and normal plate) were used to stimulate Enterobacter cloacae, and their respective pathogenicities were compared at the proteomic, mRNA and metabolomic levels. METHODS 2D-DIGE combined with MALDI-TOF-MS/MS, RT-PCR and 1H NMR were used to analyze the differential expression levels of proteins, mRNA and metabolites. RESULTS A total of 109 proteins were identified by 2D-DIGE and mass spectrometry after pairwise comparison within three culture environments, clustered into 3 classes and 183 functional categories, which were involved in 23 pathways. Based on the 2D-DIGE results, multiple proteins were selected for verification by mRNA expression. These results confirmed that most of the proteins were regulated at the transcriptional level. Thirty-eight metabolites were detected by NMR, which correlated with the differentially expressed proteins under different treatment conditions. CONCLUSIONS The results show that culture in a blood agar plate and a suitable concentration of iron promote the pathogenicity of E. cloacae and that high iron concentrations may have adverse effects on growth and iron uptake and utilization by E. cloacae.

中文翻译：

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从宁波沿岸排污口分离出的阴沟肠杆菌ENHKU01的致病性的多组学分析。

背景技术铁的获取对于细菌和血液的致病性很重要。使用三种不同的培养环境（铁刺激，血琼脂平板和普通平板）刺激阴沟肠杆菌，并在蛋白质组学，mRNA和代谢组学水平上比较了它们各自的致病性。方法采用2D-DIGE结合MALDI-TOF-MS / MS，RT-PCR和1H NMR分析蛋白质，mRNA和代谢产物的差异表达水平。结果在两个培养环境中通过成对比较，通过2D-DIGE和质谱分析共鉴定出109种蛋白质，分为3类和183个功能类别，涉及23个途径。基于2D-DIGE结果，选择了多种蛋白质以通过mRNA表达进行验证。这些结果证实，大多数蛋白质在转录水平上受到调控。通过NMR检测到38种代谢物，这些代谢物在不同处理条件下与差异表达的蛋白质相关。结论结果表明，在血琼脂平板中培养和适当浓度的铁可促进阴沟肠杆菌的致病性，高铁浓度可能会对阴沟肠杆菌的生长以及铁的吸收和利用产生不利影响。