Metastases, responsible for most of the solid tumor associated deaths, require angiogenesis and changes in endothelial cells. In this work, the effect of the secretomes of three breast tumor cell lines (MCF-7, MDA-MB-231 and ZR-75-30) on human umbilical vein endothelial cells (HUVEC) morphology was investigated. HUVEC treated with secretomes from breast cells were analyzed by confocal and time-lapse microscopy. Secretomes from ZR-75-30 and MDA-MB-231 cells modify the morphology and adhesion of HUVEC. These changes may provoke the loss of endothelial monolayer integrity. In consequence, tumor cells could have an increased access to circulation, which would then enhance metastasis.

导致大多数实体瘤相关死亡的转移灶需要血管生成和内皮细胞变化。在这项工作中，研究了三种乳腺肿瘤细胞系（MCF-7，MDA-MB-231和ZR-75-30）的分泌基因组对人脐静脉内皮细胞（HUVEC）形态的影响。通过共聚焦和延时显微镜分析了用乳腺分泌蛋白治疗的HUVEC。ZR-75-30和MDA-MB-231细胞的分泌蛋白改变了HUVEC的形态和粘附。这些变化可能会引起内皮单层完整性的丧失。因此，肿瘤细胞可能具有更多的循环途径，从而增强了转移能力。