In this study, we demonstrated an oxidative method with free radical to generate 3,5,4′-trihydroxy-trans-stilbene (trans-resveratrol) metabolites and detect sequentially by an autosampler coupling with liquid chromatography electrospray ionization tandem mass spectrometer (LC-ESI–MS/MS). In this oxidative method, the free radical initiator, ammonium persulfate (APS), was placed in a sample bottle containing resveratrol to produce oxidative derivatives, and the reaction progress was tracked by autosampler sequencing. Resveratrol, a natural product with purported cancer preventative qualities, produces metabolites including dihydroresveratrol, 3,4′-dihydroxy-trans-stilbene, lunularin, resveratrol monosulfate, and dihydroresveratrol monosulfate by free radical oxidation. Using APS free radical, the concentrations of resveratrol derivatives differ as a function of time. Besides simple, convenient and time- and labor saving, the advantages of free radical oxidative method of its in situ generation of oxidative derivatives followed by LC-ESI–MS/MS can be utilized to evaluate different metabolites in various conditions.

中文翻译：

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一种利用自由基氧化的衍生方法通过串联质谱预测白藜芦醇代谢物

在这项研究中，我们展示了一种使用自由基的氧化方法来生成 3,5,4'-三羟基-反式-二苯乙烯（反式-白藜芦醇）代谢物，并通过自动进样器与液相色谱电喷雾串联质谱仪 (LC- ESI-MS/MS）。在这种氧化方法中，将自由基引发剂过硫酸铵 (APS) 放入含有白藜芦醇的样品瓶中以产生氧化衍生物，并通过自动进样器测序跟踪反应进程。白藜芦醇是一种具有抗癌功效的天然产物，通过自由基氧化产生代谢物，包括二氢白藜芦醇、3,4'-二羟基-反式二苯乙烯、月桂甙、白藜芦醇单硫酸盐和二氢白藜芦醇单硫酸盐。使用APS自由基，白藜芦醇衍生物的浓度随时间而不同。除了简单、方便、省时省力外，自由基氧化法原位生成氧化衍生物，然后进行 LC-ESI-MS/MS 的优点可用于评估各种条件下的不同代谢物。