Simultaneous imaging of cerebral hemodynamic changes in response to functional activation during drug intoxication provides a valuable strategy to assess cocaine-induced neurovascular dysfunction. However, this requires tools with sufficient spatiotemporal resolution and an adequate signal to noise ratio (SNR). Though several technologies have been developed to address this demand during functional brain activation, their spatiotemporal resolution has been compromised to preserve SNR. In this study, we combine spatiotemporal-domain laser speckle contrast analysis and image correlation techniques to integrate the multi-wavelength spectroimaging and laser speckle contrast imaging (MW-LSCI). Experimental results show that optimized spatiotemporal resolution with enhanced SNR were achieved that enabled simultaneous measurement of multiple hemodynamic responses (i.e., ΔHbO2, ΔHbR, ΔHbT, and ΔCBF) during cocaine administration. Specifically, cocaine-induced functional cerebral hemodynamic changes were accessed by measuring the activation responses to forepaw electrical stimulation at different times after cocaine administration. With an improved spatiotemporal resolution and SNR, the system was able to differentiate the heterogeneity of cocaine's effects on the cerebral vasculature and on tissue metabolism, demonstrating the unique capability of MW-LSCI for various brain functional and pharmacological studies.

中文翻译：

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通过集成多波长光谱成像和激光散斑对比成像评估可卡因诱导的对前爪刺激的异常脑血流动力学反应

在药物中毒期间响应功能激活的脑血流动力学变化的同时成像为评估可卡因引起的神经血管功能障碍提供了一种有价值的策略。然而，这需要具有足够时空分辨率和足够信噪比 (SNR) 的工具。尽管已经开发了多种技术来解决功能性大脑激活过程中的这种需求，但它们的时空分辨率已受到影响以保持 SNR。在这项研究中，我们结合时空域激光散斑对比度分析和图像相关技术，将多波长光谱成像和激光散斑对比度成像 (MW-LSCI) 相结合。实验结果表明，实现了具有增强 SNR 的优化时空分辨率，能够在可卡因给药期间同时测量多个血流动力学反应（即，ΔHbO2、ΔHbR、ΔHbT 和 ΔCBF）。具体而言，通过测量可卡因给药后不同时间对前爪电刺激的激活反应来访问可卡因诱导的功能性脑血流动力学变化。凭借改进的时空分辨率和 SNR，该系统能够区分可卡因对脑血管系统和组织代谢影响的异质性，证明了 MW-LSCI 在各种脑功能和药理学研究中的独特能力。通过测量可卡因给药后不同时间对前爪电刺激的激活反应来评估可卡因诱导的功能性脑血流动力学变化。凭借改进的时空分辨率和 SNR，该系统能够区分可卡因对脑血管系统和组织代谢影响的异质性，证明了 MW-LSCI 在各种脑功能和药理学研究中的独特能力。通过测量可卡因给药后不同时间对前爪电刺激的激活反应来评估可卡因诱导的功能性脑血流动力学变化。凭借改进的时空分辨率和 SNR，该系统能够区分可卡因对脑血管系统和组织代谢影响的异质性，证明了 MW-LSCI 在各种脑功能和药理学研究中的独特能力。