Background

Airway epithelium is an active and important component of the immunological response in the pathophysiology of obstructive lung diseases. Recent studies suggest an important role for vitamin D₃ in asthma severity and treatment response.

Objective

Our study evaluated the influence of an active form of vitamin D₃ on the expression of selected mediators of allergic inflammation in the respiratory epithelium.

Material and Methods

Primary nasal and bronchial epithelial cells were exposed to1,25D₃ for 1 hour and were then stimulated or not with IL-4, TNF-α, LPS, and poly I:C. After 24 hours TSLP, IL-33, and IL-25 protein levels were measured in culture supernatants usingELISAandmRNAlevels in cells by real time PCR.

Results

1,25D₃ increased TSLP concentration in unstimulated nasal epithelial cells, but did not influence IL-33 and IL-25 expression. In IL-4-stimulated epithelial cell cultures 1,25D₃ mostly inhibited TSLP and IL-33 expression. In LPS-treated cultures 1,25D₃ decreased IL-33 expression. Simultaneously 1,25D₃ augmented IL-25 production in the same model of stimulation.

Conclusion

Our study revealed the dual nature of vitamin D₃ manifested in both pro- and anti-inflammatory properties observed in airway epithelial cells.

中文翻译：

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1,25-二羟基维生素D3对呼吸道上皮细胞TSLP，IL-33和IL-25表达的影响。

背景

气道上皮是阻塞性肺疾病的病理生理学中免疫应答的活跃和重要组成部分。最近的研究表明维生素D ₃在哮喘严重程度和治疗反应中起重要作用。

客观的

我们的研究评估了维生素D ₃的活性形式对呼吸道上皮中过敏性炎症所选介体表达的影响。

材料与方法

将原鼻和支气管上皮细胞暴露于1,25D ₃下1小时，然后用IL-4，TNF- α，LPS和poly I：C刺激或不刺激。24小时后，使用ELISA和细胞中的mRNA水平通过实时PCR测量培养上清液中的TSLP，IL-33和IL-25蛋白水平。

结果

1,25D ₃增加了未刺激的鼻上皮细胞中TSLP的浓度，但不影响IL-33和IL-25的表达。在IL-4刺激的上皮细胞培养物中，1,2,5D ₃主要抑制TSLP和IL-33的表达。在LPS处理的培养物中1,25D ₃降低了IL-33的表达。同时，在相同的刺激模型中1,25D ₃增加了IL-25的产生。

结论

我们的研究表明，维生素D ₃的双重性质在气道上皮细胞中观察到的促炎和抗炎特性中均得到体现。