A protocol for obtaining bulbs via in vitro organogenesis was developed for tarda tulip (Tulipa tarda Stapf). Scale explants were obtained from bulbs formed at the base of seedlings or from adventitious bulbs that developed from callus tissue forming on stolons or on germinating seeds. Some explants were subjected to chilling at 5°C for 12 wk. The culture media contained 3 or 6% sucrose and was supplemented with either no growth regulators, either 0.5 μM 6-benzyl-aminopurine (BAP) or 18.9 or 94.6 μM abscisic acid (ABA). Cultures were maintained in the dark at 20°C. Callus tissue developed mainly on media without growth regulators or with BAP. Callus was formed from up to 96% of explants derived from non-chilled adventitious bulbs that were treated with 3% sucrose and 0.5 μM BAP. Less callus was formed from chilled explants compared with non-chilled explants. Newly formed adventitious bulbs appeared on the explants via direct and indirect organogenesis. The media with BAP promoted the formation of adventitious bulbs at a rate of 56-92% from non-chilled explants, whereas a maximum rate of 36% was observed from chilled explants. ABA inhibited the induction of adventitious bulbs and callus. The adventitious bulbs obtained in these experiments contained a meristem, which was evidence that they had developed properly.

中文翻译：

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在源自种子的外植体的塔达郁金香（Tulipa tarda Stapf。）的体外培养中诱导鳞茎器官发生。

通过体外器官发生获得球茎的方案已被开发用于塔尔达郁金香（Tulipa tarda Stapf）。鳞片外植体是从幼苗基部形成的鳞茎或从from茎或发芽种子上形成的愈伤组织形成的不定鳞茎获得的。一些外植体在5℃下冷冻12周。培养基中含有3或6％的蔗糖，并补充有没有生长调节剂的0.5μM6-苄基氨基嘌呤（BAP）或18.9或94.6μM脱落酸（ABA）。将培养物在20℃下保持在黑暗中。愈伤组织主要在无生长调节剂或BAP的培养基上发育。愈伤组织是由多达96％的外植体形成的，这些外植体是用3％蔗糖和0.5μMBAP处理的非冷藏不定鳞茎。与非冷冻外植体相比，冷冻外植体形成的愈伤组织更少。通过直接和间接器官发生，新形成的不定鳞茎出现在外植体上。含BAP的培养基以非冷冻外植体促进不定鳞球形成的比率为56-92％，而从冷冻外植体中观察到的最大比率为36％。ABA抑制不定鳞茎和愈伤组织的诱导。在这些实验中获得的不定鳞鳞茎含有一个分生组织，这证明它们已经发育适当。ABA抑制不定鳞茎和愈伤组织的诱导。在这些实验中获得的不定鳞鳞茎含有一个分生组织，这证明它们已经发育适当。ABA抑制不定鳞茎和愈伤组织的诱导。在这些实验中获得的不定鳞鳞茎含有一个分生组织，这证明它们已经发育适当。