Multifocal multiphoton laser scanning microscopy (mfMPLSM) in the biological and medical sciences has the potential to become a ubiquitous tool for obtaining high-resolution images at video rates. While current implementations of mfMPLSM achieve very high frame rates, they are limited in their applicability to essentially those biological samples that exhibit little or no scattering. In this paper, we report on a method for mfMPLSM in which whole-field detection with a single detector, rather than detection with a matrix of detectors, such as a charge-coupled device (CCD) camera, is implemented. This advance makes mfMPLSM fully compatible for use in imaging through scattering media. Further, we demonstrate that this method makes it possible to simultaneously obtain multiple images and view differences in excitation parameters in a single scan of the specimen.

中文翻译：

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差分多光子激光扫描显微镜

生物和医学科学中的多焦点多光子激光扫描显微镜 (mfMPLSM) 有可能成为以视频速率获取高分辨率图像的无处不在的工具。虽然 mfMPLSM 的当前实现实现了非常高的帧速率，但它们的适用性基本上仅限于那些表现出很少或没有散射的生物样本。在本文中，我们报告了一种用于 mfMPLSM 的方法，其中使用单个检测器进行全场检测，而不是使用诸如电荷耦合器件 (CCD) 相机等检测器矩阵进行检测。这一进步使 mfMPLSM 完全兼容用于通过散射介质成像。更多，