A new cryomacroscope prototype-a visualization device for the in situ analysis of cryopreserved biological samples-is presented in the current study. In order to visualize samples larger than the field of view of the optical setup, a scanning mechanism is integrated into the system, which represents a key improvement over previous cryomacroscope prototypes. Another key feature of the new design is in its compatibility with available top-loading controlled-rate cooling chambers, which eliminates the need for a dedicated cooling mechanism. The objective for the current development is to create means to generate a single digital movie of an experimental investigation, with all relevant data overlaid. The visualization capabilities of the scanning cryomacroscope are demonstrated in the current study on the cryoprotective agent dimethyl sulfoxide and the cryoprotective cocktail DP6. Demonstrated effects include glass formation, various regimes of crystallization, thermal contraction, and fracture formation.

中文翻译：

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扫描冷冻宏观镜——用于冷冻保存研究的设备原型


当前的研究中提出了一种新的冷冻宏观镜原型——一种用于冷冻保存生物样本原位分析的可视化装置。为了可视化大于光学装置视场的样品，系统中集成了扫描机制，这代表了对之前冷冻宏观显微镜原型的关键改进。新设计的另一个关键特点是它与可用的顶部装载控制速率冷却室兼容，从而无需专用的冷却机制。当前开发的目标是创建一种方法来生成实验调查的单个数字电影，并覆盖所有相关数据。目前对冷冻保护剂二甲亚砜和冷冻保护混合物 DP6 的研究证明了扫描冷冻宏观镜的可视化功能。已证实的效应包括玻璃形成、各种结晶状态、热收缩和断裂形成。