In this study the effect of freeze drying on the microstructure, texture, and tenderness of Semitendinous and Gluteus Medius bovine muscles were analyzed applying Scanning Electron Microscopy combined with image analysis. Samples were analyzed by Scanning Electron Microscopy at different magnifications (250, 500, and 1,000×). Texture parameters were analyzed by Texture analyzer and by image analysis. Tenderness by Warner-Bratzler shear force. Significant differences (p < 0.05) were obtained for image and instrumental texture features. A linear trend with a linear correlation was applied for instrumental and image features. Image texture features calculated from Gray Level Co-occurrence Matrix (homogeneity, contrast, entropy, correlation and energy) at 1,000× in both muscles had high correlations with instrumental features (chewiness, hardness, cohesiveness, and springiness). Tenderness showed a positive correlation in both muscles with image features (energy and homogeneity). Combing Scanning Electron Microscopy with image analysis can be a useful tool to analyze quality parameters in meat.Summary SCANNING 38:727-734, 2016. © 2016 Wiley Periodicals, Inc.

中文翻译：

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扫描电子显微镜结合图像处理技术：分析牛半腱和臀中肌的微观结构、质地和压痛

在这项研究中，应用扫描电子显微镜结合图像分析，分析了冷冻干燥对牛半腱和臀中肌的微观结构、质地和柔软度的影响。通过扫描电子显微镜在不同的放大倍数（250、500 和 1,000 倍）下分析样品。通过纹理分析器和图像分析来分析纹理参数。Warner-Bratzler 剪切力的柔软度。图像和仪器纹理特征获得了显着差异（p < 0.05）。具有线性相关性的线性趋势应用于仪器和图像特征。从灰度共生矩阵（均匀性、对比度、熵、相关性和能量）计算的图像纹理特征在两块肌肉中 1,000 倍与仪器特征（咀嚼度、硬度、凝聚力和弹性）。柔软度在两种肌肉中均与图像特征（能量和均匀性）呈正相关。将扫描电子显微镜与图像分析相结合可以成为分析肉类质量参数的有用工具。摘要扫描 38:727-734, 2016. © 2016 Wiley Periodicals, Inc.