Increasing resistance to conventional antibiotics among microorganisms is one of the leading problems of medicine nowadays. Antimicrobial peptides are compounds exhibiting both antibacterial and antifungal activities. However, it is difficult to predict whether a designed new compound would exhibit any biological activity. Moreover, purification of the peptides is one of the most time-consuming and expensive steps of the synthesis that sometimes leads to unnecessary loss of solvents and reagents. In our study we have developed a thin-layer chromatography (TLC) direct bioautography technique for rapid determination of antimicrobial activity of peptides without the necessity of high-performance liquid chromatography purification. In this assay, crude peptides were applied and separated on a TLC plate. Then, pre-prepared plates were dipped into microbial suspension and incubated under optimum conditions for bacteria and fungi as well. The activity of the tested compounds was visualized by spraying the TLC plates with a cell viability reagent, resazurin (7-hydroxy-3H-phenoxazin-3-one 10-oxide). Effectiveness of this assay was compared with minimal inhibitory concentration results obtained by broth microdilution assay. Interestingly, so far such a screening method has not been applied for this group of compounds.

中文翻译：

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快速筛选抗菌合成肽。

如今，微生物对常规抗生素的抗药性增加是医学的主要问题之一。抗菌肽是同时具有抗菌和抗真菌活性的化合物。但是，很难预测所设计的新化合物是否会表现出任何生物学活性。此外，肽的纯化是合成中最耗时和最昂贵的步骤之一，有时会导致不必要的溶剂和试剂损失。在我们的研究中，我们开发了薄层色谱（TLC）直接生物自显影技术，可快速测定肽的抗菌活性，而无需进行高效液相色谱纯化。在该测定中，将粗肽施加并在TLC板上分离。然后，将预先准备的板浸入微生物悬浮液中，并在最佳条件下孵育细菌和真菌。通过用细胞生存力试剂刃天青（7-羟基-3H-苯恶嗪-3-酮10-氧化物）喷洒TLC板来可视化所测试化合物的活性。将该测定的有效性与通过肉汤微量稀释测定获得的最小抑菌浓度结果进行了比较。有趣的是，到目前为止，这种筛选方法尚未应用于这组化合物。将该测定的有效性与通过肉汤微量稀释测定获得的最小抑菌浓度结果进行了比较。有趣的是，到目前为止，这种筛选方法尚未应用于这组化合物。将该测定的有效性与通过肉汤微量稀释测定获得的最小抑菌浓度结果进行了比较。有趣的是，到目前为止，这种筛选方法尚未应用于这组化合物。