Single-molecule fluorescence microscopy techniques can be used in combination with micrometer length-scale temperature control and Förster resonance energy transfer (FRET) in order to gain detailed information about fundamental biophysical phenomena. In particular, this combination of techniques has helped foster the development of remarkable quantitative tools for studying both time- and temperature-dependent structural kinetics of biopolymers. Over the past decade, multiple research efforts have successfully incorporated precise spatial and temporal control of temperature into single-molecule FRET (smFRET)-based experiments, which have uncovered critical thermodynamic information on a wide range of biological systems such as conformational dynamics of nucleic acids. This review provides an overview of various temperature-dependent smFRET approaches from our laboratory and others, highlighting efforts in which such methods have been successfully applied to studies of single-molecule nucleic acid folding.

中文翻译：

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温度依赖性单分子Förster共振能量转移的生物物理见解。

单分子荧光显微镜技术可与微米级长度标度温度控制和福斯特共振能量转移（FRET）结合使用，以获取有关基本生物物理现象的详细信息。尤其是，这种技术组合帮助促进了卓越的定量工具的开发，以研究生物聚合物的时间和温度相关的结构动力学。在过去的十年中，多项研究工作已成功地将温度的精确时空控制纳入基于单分子FRET（smFRET）的实验中，该实验发现了有关广泛生物系统的关键热力学信息，例如核酸的构象动力学。 。