Immunogenetic data from wild primate populations have been difficult to obtain, due to logistic and methodological constraints. We applied a well-characterized deep sequencing method for MHC I typing, developed for macaques, to a population of wild red colobus to assess the feasibility of identifying MHC I-A/B haplotypes. Ten individuals produced sufficient data from blood and tissue samples to assign haplotypes. Eighty-two sequences were classified as red colobus MHC I alleles distributed across six MHC I loci. Individuals averaged ~13k reads across six MHC I loci, with 83% of all alleles representing either MHC I-A or MHC I-B loci. This study not only represents an important advance in the identification and genotyping of MHC in the endangered red colobus but also shows the potential for using this approach in other endangered wild primates.

中文翻译：

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在濒危的旧世界猴子中使用深度测序快速鉴定主要的组织相容性复杂的I类单倍型。

由于后勤和方法上的限制，很难从野生灵长类种群获得免疫原性数据。我们将针对猕猴开发的针对MHC I分型的功能完备的深度测序方法应用于野生红疣猴，以评估鉴定MHC IA / B单倍型的可行性。十个人从血液和组织样本中获得了足够的数据来指定单倍型。82个序列被分类为分布在六个MHC I基因座上的红色疣猴MHC I等位基因。个体在六个MHC I基因座上的平均阅读量约为13k，其中所有等位基因的83％代表MHC IA或MHC IB基因座。这项研究不仅代表了在濒临灭绝的红色疣猴中MHC的鉴定和基因分型的重要进展，而且还表明了在其他濒临灭绝的野生灵长类动物中使用这种方法的潜力。