ABSTRACT Invasion of melanoma cells from the primary tumor involves interaction with adjacent tissues and extracellular matrix. The extent of this interaction is not fully understood. In this study Raman spectroscopy was applied to cryo-sections of established 3D models of melanoma in human skin. Principal component analysis was used to investigate differences between the tumor and normal tissue and between the peri-tumor area and the normal skin. Two human melanoma cells lines A375SM and C8161 were investigated and compared in 3D melanoma models. Changes were found in protein conformations and tryptophan configurations across the entire melanoma samples, in tyrosine orientation and in more fluid lipid packing only in tumor dense areas, and in increased glycogen content in the peri-tumor areas of melanoma. Raman spectroscopy revealed changes around the perimeter of a melanoma tumor as well as detecting differences between the tumor and the normal tissue.

中文翻译：

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拉曼光谱使用组织工程黑色素瘤模型检测黑色素瘤和黑色素瘤周围的组织

摘要 从原发性肿瘤侵入黑色素瘤细胞涉及与邻近组织和细胞外基质的相互作用。这种相互作用的程度尚不完全清楚。在这项研究中，拉曼光谱应用于已建立的人体皮肤黑色素瘤 3D 模型的冷冻切片。主成分分析用于研究肿瘤与正常组织之间以及肿瘤周围区域与正常皮肤之间的差异。在 3D 黑色素瘤模型中研究和比较了两种人类黑色素瘤细胞系 A375SM 和 C8161。在整个黑色素瘤样本中发现了蛋白质构象和色氨酸构型的变化，酪氨酸方向和仅在肿瘤密集区域中更流动的脂质堆积，以及黑色素瘤肿瘤周围区域的糖原含量增加。