To investigate the feasibility and efficacy of a virus-like particle (VLP) vaccine composed of the conserved antigenic epitopes of respiratory syncytial virus (RSV), the chimeric RSV VLPs HBcΔ-tG and HBcΔ-tG/M282-90 were generated based on the truncated hepatitis B virus core protein (HBcΔ). HBcΔ-tG consisted of HBcΔ, the conserved region (aa 144-204) of the RSV G protein. HBcΔ-tG was combined with a single peptide (aa 82-90) of the M2 protein to generate HBcΔ-tG/M282-90. Immunization of mice with the HBcΔ-tG or HBcΔ-tG/M282-90 VLPs elicited RSV-specific IgG and neutralizing antibody production and conferred protection against RSV infection. Compared with HBcΔ-tG, HBcΔ-tG/M282-90 induced decreased Th2 cytokine production (IL-4 and IL-5), increased Th1 cytokine response (IFN-γ, TNF-α, and IL-2), and increased ratios of IgG2a/IgG1 antibodies, thereby relieving pulmonary pathology upon subsequent RSV infection. Our results demonstrated that chimeric HBcΔ-tG/M282-90 VLPs represented an effective RSV subunit vaccine candidate.

中文翻译：

---

包含G蛋白保守区和M2蛋白单个肽的嵌合病毒样颗粒可预防呼吸道合胞病毒感染。

为了研究由呼吸道合胞病毒（RSV）的保守抗原表位组成的病毒样颗粒（VLP）疫苗的可行性和功效，基于该抗体产生了嵌合RSV VLPHBcΔ-tG和HBcΔ-tG/ M282-90。截短的乙型肝炎病毒核心蛋白（HBcΔ）。HBcΔ-tG由HBcΔ（RSV G蛋白的保守区（aa 144-204））组成。HBcΔ-tG与M2蛋白的单个肽（aa 82-90）结合生成HBcΔ-tG/ M282-90。用HBcΔ-tG或HBcΔ-tG/ M282-90 VLP免疫小鼠会引起RSV特异性IgG，并中和抗体产生并赋予针对RSV感染的保护。与HBcΔ-tG相比，HBcΔ-tG/ M282-90诱导的Th2细胞因子产生减少（IL-4和IL-5），Th1细胞因子应答增加（IFN-γ，TNF-α和IL-2），并且比率增加IgG2a / IgG1抗体，从而减轻随后的RSV感染引起的肺部疾病。我们的结果表明，嵌合的HBcΔ-tG/ M282-90 VLP代表了有效的RSV亚基疫苗候选者。