Metabolomics is the study of metabolites present in biological samples such as biofluids, tissue/cellular extracts and culture media. Measurement of these metabolites is achieved through use of analytical techniques such as NMR and mass spectrometry coupled to liquid chromatography. Combining metabolomic data with multivariate data analysis tools allows the elucidation of alterations in metabolic pathways under different physiological conditions. Applications of NMR-based metabolomics have grown in recent years and it is now widely used across a number of disciplines. The present review gives an overview of the developments in the key steps involved in an NMR-based metabolomics study. Furthermore, there will be a particular emphasis on the use of NMR-based metabolomics in nutrition research.

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基于 NMR 的代谢组学：从样品制备到营养研究中的应用

代谢组学是研究生物样品中存在的代谢物，例如生物流体、组织/细胞提取物和培养基。这些代谢物的测量是通过使用分析技术（如 NMR 和质谱联用液相色谱法）来实现的。将代谢组学数据与多变量数据分析工具相结合，可以阐明不同生理条件下代谢途径的变化。近年来，基于 NMR 的代谢组学的应用不断增长，现已广泛应用于多个学科。本综述概述了基于 NMR 的代谢组学研究中涉及的关键步骤的发展。此外，将特别强调在营养研究中使用基于核磁共振的代谢组学。