We present a real-time multimodal near-infrared imaging technology that tracks externally-induced axial motion of magnetic microbeads in single cells in culture. The integrated multimodal imaging technique consists of phase-sensitive magnetomotive optical coherence microscopy (MM-OCM) and multiphoton microscopy (MPM). MPM is utilized for the visualization of multifunctional fluorescent and magnetic microbeads, while MM-OCM detects, with nanometer-scale sensitivity, periodic displacements of the microbeads induced by the modulation of an external magnetic field. Magnetomotive signals are measured from mouse macrophages, human breast primary ductal carcinoma cells, and human breast epithelial cells in culture, and validated with full-field phase-sensitive microscopy. This methodology demonstrates the capability for imaging controlled cell dynamics and has the potential for measuring cell biomechanical properties, which are important in assessing the health and pathological state of cells.

中文翻译：

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用于跟踪磁微珠和细胞力学的高分辨率相敏磁动光学相干显微镜


我们提出了一种实时多模态近红外成像技术，可跟踪培养物中单细胞中磁性微珠的外部诱导轴向运动。集成多模态成像技术由相敏磁动光学相干显微镜（MM-OCM）和多光子显微镜（MPM）组成。 MPM 用于多功能荧光和磁性微珠的可视化，而 MM-OCM 以纳米级灵敏度检测由外部磁场调制引起的微珠的周期性位移。从培养的小鼠巨噬细胞、人乳腺原发性导管癌细胞和人乳腺上皮细胞中测量磁动信号，并通过全视场相敏显微镜进行验证。该方法展示了对受控细胞动力学进行成像的能力，并具有测量细胞生物力学特性的潜力，这对于评估细胞的健康和病理状态非常重要。