To assess the prevalence and occurrence of eleven periodontopathogens in subgingival biofilm of banded and bonded molars during the first period of fixed orthodontic treatment. Subjects were selected from patients referred to orthodontic treatment and were divided in two groups: group A comprised fifteen patients (14.4±2.45 years of age) who received orthodontic bands on first permanent molars and group B of ten patients (15.7±1.87 years of age) with directly bonded tubes on the labial surface of the same teeth. Subgingival sample collection was performed before bands and tubes application and 4-7 weeks after attachment placement. DNA-strip tehnique was used to assess the presence of eleven putative periodontopathogens at each time point. Fusobacterium nucleatum, Eikenella corrodens and Capnocytophaga spp. were found in a large number of samples, other periodontopathogens were present in a smaller rate. The 4-7 weeks after attachment placement a slight increase of putative species was observed in both groups. The presence of orthodontic tubes and bands influence the accumulation and composition of subgingival microbiota. Higher level of oral hygiene should be achieved before and during orthodontic treatment in order to prevent any side effects on periodontal tissues.

中文翻译：

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在固定正畸治疗早期评估带状和粘结性磨牙的龈下生物膜中的牙周病原体。

评估在固定正畸治疗的第一阶段中，带状和粘结性磨牙的龈下生物膜中十一种牙周病原体的患病率和发生情况。受试者从接受正畸治疗的患者中选出，分为两组：A组包括15名（14.4±2.45岁）在第一恒磨牙上接受正畸带治疗的患者，B组则是10例（15.7±1.87岁）的患者），并在同一颗牙齿的唇面上直接粘合管。牙龈下样品的采集是在应用条带和输液管之前以及在放置附件后的4-7周进行的。使用DNA条带技术在每个时间点评估十一种推定的牙周病原体的存在。核梭状芽胞杆菌，腐蚀的艾肯氏菌和Capnocytophaga spp。在大量样本中被发现 其他牙周病原体的发生率较低。附着放置后4-7周，两组均观察到推定的物种略有增加。正畸管和正畸带的存在影响龈下菌群的​​积累和组成。在正畸治疗之前和期间，应达到更高的口腔卫生水平，以防止对牙周组织产生任何副作用。