Interferon-gamma (IFN-γ) and P2X7 receptor are crucial for host defence against mycobacterial infections. Recent studies have indicated that IFN-γ, IFN-γ receptor 1 (IFN-γR1) andP2X7 gene polymorphisms are associated with susceptibility to pulmonary tuberculosis (TB). However, the relationship between IFN-γ and P2X7 polymorphism and TB susceptibility remains inconclusive in Iranian population. For this reason, single nucleotide polymorphisms (SNPs) in IFN-γ (G+2109A), IFN-γR1 (G-611A) and P2X7 genes (at -762, 1513 position) in patients (n = 100) were assessed using PCR-RFLP. Data were analysed with SPSS version 18. For the 2109 loci of IFN-γ gene, the frequency of mutant alleles between patients and controls were not statistically significant. However, there was a significant difference between the TB patient and controls for -611 alleles of IFN-γR1 (P = 0.01). Additionally, the frequency of P2X7 gene polymorphisms (SNP-762 and 1513) between patients and controls was statistically significant. In conclusions, our study revealed a significant association of IFN-γR1 and P2X7 genes polymorphisms with risk of developing TB in Iranian population.

中文翻译：

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伊朗患者中 IFN-γ 和 P2X7 受体基因多态性与结核病易感性的关联。


干扰素-γ (IFN-γ) 和 P2X7 受体对于宿主防御分枝杆菌感染至关重要。近年来研究表明，IFN-γ、IFN-γ受体1（IFN-γR1）和P2X7基因多态性与肺结核（TB）易感性相关。然而，伊朗人群中 IFN-γ 和 P2X7 多态性与结核病易感性之间的关系仍无定论。因此，使用 PCR 评估了患者 (n = 100) 的 IFN-γ (G+2109A)、IFN-γR1 (G-611A) 和 P2X7 基因（-762、1513 位点）的单核苷酸多态性 (SNP) -RFLP。使用SPSS 18版对数据进行分析。对于IFN-γ基因的2109个位点，患者和对照之间突变等位基因的频率没有统计学意义。然而，结核病患者和对照者之间的 IFN-γR1 -611 等位基因存在显着差异（P = 0.01）。此外，患者和对照组之间 P2X7 基因多态性（SNP-762 和 1513）的频率具有统计学意义。总之，我们的研究揭示了 IFN-γR1 和 P2X7 基因多态性与伊朗人群患结核病的风险存在显着关联。