Replication of positive-strand RNA viruses occurs in tight association with reorganized host cell membranes. In a concerted fashion, viral and cellular factors generate distinct organelle-like structures, designated viral replication factories. These virus-induced compartments promote highly efficient genome replication, allow spatiotemporal coordination of the different steps of the viral replication cycle, and protect viral RNA from the hostile cytoplasmic environment. The combined use of ultrastructural and functional studies has greatly increased our understanding of the architecture and biogenesis of viral replication factories. Here, we review common concepts and distinct differences in replication organelle morphology and biogenesis within the Flaviviridae family, exemplified by dengue virus and hepatitis C virus. We discuss recent progress made in our understanding of the complex interplay between viral determinants and subverted cellular membrane homeostasis in biogenesis and maintenance of replication factories of this virus family.

中文翻译：

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黄病毒科复制细胞器：哦，编织的网有多复杂。

正链RNA病毒的复制与重组的宿主细胞膜紧密结合。以协调一致的方式，病毒和细胞因子产生不同的细胞器样结构，称为病毒复制工厂。这些病毒诱导的区室可促进高效的基因组复制，允许时空协调病毒复制周期的不同步骤，并保护病毒RNA免受有害细胞质环境的侵害。超结构和功能研究的结合使用极大地增加了我们对病毒复制工厂的结构和生物发生的理解。在这里，我们审查了共同的概念和黄病毒科内复制细胞器形态和生物发生方面的明显差异，以登革热病毒和丙型肝炎病毒为例。