Cellular entry of retroviruses is the first critical stage of retroviral replication. Live cell imaging has been utilized to visualize the dynamics, localization, and kinetics of the viral fusion process. Here, we review the different methodologies used for live cell imaging and how the use of these techniques has better elucidated the viral entry process of avian sarcoma and leukosis virus (ASLV) and human immunodeficiency virus type 1 (HIV-1) as well as cell-to-cell transmission of retroviruses. Although some controversies remain, further development of these techniques will provide new insights into the process and dynamics of retroviral fusion in vivo.

中文翻译：

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逆转录病毒进入的活细胞成像。

逆转录病毒的细胞进入是逆转录病毒复制的第一个关键阶段。活细胞成像已用于可视化病毒融合过程的动力学，定位和动力学。在这里，我们回顾了用于活细胞成像的不同方法，以及这些技术的使用如何更好地阐明了禽肉瘤和白血病病毒（ASLV）和1型人类免疫缺陷病毒（HIV-1）以及细胞的病毒进入过程逆转录病毒的细胞间传播。尽管仍然存在一些争议，但是这些技术的进一步发展将为体内逆转录病毒融合的过程和动力学提供新的见解。