This study evaluated the breath CH₄ excretion and concentration of M. smithii in intestinal microbiota of schoolchildren from 2 slums. One hundred and eleven children from a slum near a sanitary landfill, 35 children of a slum located away from the sanitary landfill, and 32 children from a high socioeconomic level school were included in the study. Real-time PCR was performed to quantify the M. smithii nifH gene and it was present in the microbiota of all the participating children, with higher concentrations in those who lived in the slum near the landfill ( CFU/g of feces), comparing with the children from the slum away from the landfill ( CFU/g of feces) and those from the high socioeconomic level group ( CFU/g of feces). The prevalence of children who present breath methane was 53% in the slum near the landfill, 31% in the slum further away from the landfill and, 22% in the high socioeconomic level group. To live near a landfill is associated with higher concentrations of M. smithii in intestinal microbiota, comparing with those who live away from the landfill, regardless of their socioeconomics conditions.

中文翻译：

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生活在卫生垃圾填埋场附近的儿童肠道菌群中的甲烷和史密斯甲烷细菌增加。

这项研究评估了来自2个贫民窟的学童肠道微生物群中呼吸道CH _4的排泄和史密斯氏菌的浓度。该研究包括一个来自卫生垃圾填埋场附近的贫民窟的11个孩子，一个远离卫生垃圾填埋场的贫民窟的35个孩子和来自社会经济水平较高的学校的32个孩子。实时荧光定量PCR定量了史密斯氏菌nifH基因，该基因存在于所有参与研究的儿童的微生物区系中，与垃圾填埋场附近的贫民区居民相比，其浓度更高（ CFU / g粪便），与远离垃圾填埋场的贫民窟中的孩子（ 每克粪便CFU）和来自社会经济高水平群体的孩子（ CFU /克粪便）。在填埋场附近的贫民窟中，出现呼吸甲烷的儿童的患病率为53％，在远离填埋场的贫民窟中占31％，在社会经济水平较高的人群中为22％。与居住在垃圾填埋场附近的人相比，无论其社会经济条件如何，与生活在垃圾填埋场附近的人相比，生活在垃圾填埋场中的史密斯氏菌在肠道菌群中的浓度都较高。