Immunoassays based on monoclonal antibodies (mAbs) are highly sensitive for the detection of polycyclic aromatic hydrocarbons (PAHs) and can be employed to determine concentrations in near real-time. A sensitive generic mAb against PAHs, named as 2G8, was developed by a three-step screening procedure. It exhibited nearly uniformly high sensitivity against 3-ring to 5-ring unsubstituted PAHs and their common environmental methylated PAHs, with IC50 values between 1.68-31 μg/L (ppb). 2G8 has been successfully applied on the KinExA Inline Biosensor system for quantifying 3-5 ring PAHs in aqueous environmental samples. PAHs were detected at a concentration as low as 0.2 μg/L. Furthermore, the analyses only required 10 min for each sample. To evaluate the accuracy of the 2G8-based biosensor, the total PAH concentrations in a series of environmental samples analyzed by biosensor and GC-MS were compared. In most cases, the results yielded a good correlation between methods. This indicates that generic antibody 2G8 based biosensor possesses significant promise for a low cost, rapid method for PAH determination in aqueous samples.

中文翻译：

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基于高灵敏度单克隆抗体的生物传感器，用于定量水性环境样品中的3-5个环多环芳烃（PAH）。

基于单克隆抗体（mAb）的免疫分析对多环芳烃（PAH）的检测高度敏感，可用于近实时测定浓度。通过三步筛选程序开发了一种针对PAHs的敏感通用mAb，名为2G8。它对3环至5环未取代的PAH及其常见的环境甲基化PAH表现出几乎一致的高灵敏度，IC50值在1.68-31μg/ L（ppb）之间。2G8已成功应用于KinExA在线生物传感器系统中，用于定量水性环境样品中的3-5个环PAH。检测到的PAHs浓度低至0.2μg/ L。此外，每个样品的分析仅需10分钟。要评估基于2G8的生物传感器的准确性，比较了通过生物传感器和GC-MS分析的一系列环境样品中的总PAH浓度。在大多数情况下，结果在方法之间产生了良好的相关性。这表明基于通用抗体2G8的生物传感器具有低成本，快速测定水性样品中PAH的方法的重大前景。