The present study focused on fermentative butyric acid production by Clostridium tyrobutyricum from pre-treated and hydrolysed wheat straw (PHWS) based on continuous operation mode and in situ acids extraction by reverse electro enhanced dialysis (REED). Different dilutions of PHWS in a synthetic medium (60–100 % v/v) were tested. It was found that continuous fermentation of PHWS greatly enhanced the sugar consumption rates and butyric acid productivity compared to batch tests, while application of REED enhanced them even further. Specifically, applying combined continuous operation mode and REED system for the fermentation of 70 % PHWS resulted in 19- and 53-fold higher glucose (1.37 g L⁻¹ h⁻¹) and xylose (0.80 g L⁻¹ h⁻¹) consumption rates, respectively, compared to those obtained by batch processing. Fermentation of 100 % PHWS continued unhindered with just urea and K₂HPO₄ added with butyric acid production rate, yield and selectivity being 1.30 g L⁻¹ h⁻¹, 0.45 g g⁻¹ sugars and 0.88 g g⁻¹ acids, respectively. These results were also confirmed in a 20 L pilot plant bioreactor system.

中文翻译：

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酪丁酸梭菌在原位去除酸的条件下连续发酵麦草水解液。

本研究的重点是在连续操作模式和逆电增强渗析（REED）的基础上，从预处理和水解的小麦秸秆（PHWS）中提取酪氨酸梭状芽孢杆菌发酵性丁酸。测试了在合成培养基（60-100％v / v）中PHWS的不同稀释度。已发现，与批次试验相比，PHWS的连续发酵极大地提高了糖消耗率和丁酸生产率，而REED的应用甚至进一步提高了糖消耗率和丁酸生产率。具体而言，将连续运行模式和REED系统组合用于70％PHW​​S的发酵，可产生19倍和53倍的葡萄糖（1.37 g L ^-1  h ^-1）和木糖（0.80 g L ^-1  h）^-1）消耗率，与通过批处理获得的消耗率相比。100％的PHWS继续发酵不受阻碍地与刚尿素和K ₂ HPO ₄添加丁酸生产速率，产率和选择性是1.30克大号^-1  ħ ^-1，0.45克克^-1的糖和0.88克克^-1，酸分别。在20 L中试植物生物反应器系统中也证实了这些结果。