String-of-beads polypeptides allow convenient delivery of epitope-based vaccines. The success of a polypeptide relies on efficient processing: constituent epitopes need to be recovered while avoiding neo-epitopes from epitope junctions. Spacers between epitopes are employed to ensure this, but spacer selection is non-trivial.We present a framework to determine optimally the length and sequence of a spacer through multi-objective optimization for human leukocyte antigen class I restricted polypeptides. The method yields string-of-bead vaccines with flexible spacer lengths that increase the predicted epitope recovery rate fivefold while reducing the immunogenicity from neo-epitopes by 44% compared to designs without spacers.

中文翻译：

---

设计带有最佳间隔子的串珠疫苗。

珠串多肽允许方便地递送基于表位的疫苗。多肽的成功取决于有效的加工：需要恢复组成的表位，同时避免表位连接处出现新表位。表位之间的间隔基可用于确保这一点，但间隔基的选择并非易事。我们提出了一个框架，可通过对人白细胞抗原I类限制性多肽进行多目标优化来最佳地确定间隔基的长度和序列。与没有间隔子的设计相比，该方法可产生具有灵活间隔子长度的串珠状疫苗，从而将预测的表位回收率提高五倍，同时使来自新表位的免疫原性降低44％。