OBJECTIVES During lung lobectomy, the operated lung completely collapses with simultaneous hypoxic pulmonary vasoconstriction, followed by expansion and reperfusion. Here, we investigated glutathione oxidation and lipoperoxidation in patients undergoing lung lobectomy, during one-lung ventilation (OLV) and after resuming two-lung ventilation (TLV), and examined the relationship with OLV duration. METHODS We performed a single-centre, observational, prospective study in 32 patients undergoing lung lobectomy. Blood samples were collected at five time-points: T0, pre-operatively; T1, during OLV, 5 minutes before resuming TLV; and T2, T3, and T4, respectively, 5, 60, and 180 minutes after resuming TLV. Samples were tested for reduced glutathione (GSH), oxidized glutathione (GSSG), glutathione redox potential, and malondialdehyde (MDA). RESULTS GSSG and MDA blood levels increased at T1, and increased further at T2. OLV duration directly correlated with marker levels at T1 and T2. Blood levels of GSH and glutathione redox potential decreased at T1-T3. GSSG, oxidized glutathione/total glutathione ratio, and MDA levels were inversely correlated with arterial blood PO2/FiO2 at T1 and T2. DISCUSSION During lung lobectomy and OLV, glutathione oxidation, and lipoperoxidation marker blood levels increase, with further increases after resuming TLV. Oxidative stress degree was directly correlated with OLV duration, and inversely correlated with arterial blood PO2/FiO2.

中文翻译：

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肺叶切除术中谷胱甘肽氧化与单肺通气时间和 PO2/FiO2 比值相关。

目的 在肺叶切除术中，手术后的肺完全塌陷，同时缺氧性肺血管收缩，随后扩张和再灌注。在这里，我们研究了接受肺叶切除术的患者、单肺通气 (OLV) 期间和恢复双肺通气 (TLV) 后的谷胱甘肽氧化和脂质过氧化，并检查了与 OLV 持续时间的关系。方法 我们对 32 名接受肺叶切除术的患者进行了一项单中心、观察性、前瞻性研究。在五个时间点采集血样：T0，术前；T0，术前；T1，OLV期间，恢复TLV前5分钟；T2、T3 和 T4 分别为恢复 TLV 后 5、60 和 180 分钟。测试了样品的还原型谷胱甘肽 (GSH)、氧化型谷胱甘肽 (GSSG)、谷胱甘肽氧化还原电位和丙二醛 (MDA)。结果 GSSG 和 MDA 血液水平在 T1 时升高，并在 T2 时进一步升高。OLV 持续时间与 T1 和 T2 的标记物水平直接相关。T1-T3 时血液中 GSH 和谷胱甘肽氧化还原电位水平下降。GSSG、氧化型谷胱甘肽/总谷胱甘肽比值和 MDA 水平与 T1 和 T2 时动脉血 PO2/FiO2 呈负相关。讨论 在肺叶切除术和 OLV 期间，谷胱甘肽氧化和脂质过氧化标志物血液水平升高，恢复 TLV 后进一步升高。氧化应激程度与OLV持续时间正相关，与动脉血PO2/FiO2负相关。