The aim of our research was to explore the most cost-efficient and optimal medium composition for the production of lipase from Pseudomonas fluorescens (NRLL B-2641) culture grown on sunflower oil cake (SuOC) by applying response surface methodology (RSM). The oil cake was used instead of carbon sources. Peptone, ammonium sulphate and the carbon source (SuOC) were the most important factors as it is obligatory for microbial growth. Subsequently, the optimum values for the carbon source, peptone and ammonium sulphate were found to be 11.10% (w/v), 1.18% (w/v) and 0.83% (w/v), respectively. Experiments carried out under optimum conditions revealed a maximum lipase activity of 10.8 U mL−1, which was achieved after 48 h of fermentation. The obtained results were finally verified with batch experiments carried out under the optimum conditions evaluated and it was demonstrated that the SuOC from agro-industrial residue as substrates can be used as an inexpensive base (carbon source) for the production of lipase by P. fluorescens (NRLL B-2641).

中文翻译：

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使用响应面法优化荧光假单胞菌 (NRLL B-2641) 在农业工业残留物培养基上的脂肪酶生产


我们研究的目的是通过应用响应面法 (RSM) 探索在葵花籽油饼 (SuOC) 上生长的荧光假单胞菌 (NRLL B-2641) 培养物生产脂肪酶的最具成本效益和最佳的培养基组成。使用油饼代替碳源。蛋白胨、硫酸铵和碳源 (SuOC) 是最重要的因素，因为它是微生物生长所必需的。随后发现碳源、蛋白胨和硫酸铵的最佳值分别为11.10% (w/v)、1.18% (w/v) 和0.83% (w/v)。在最佳条件下进行的实验显示，发酵 48 小时后，脂肪酶的最大活性为 10.8 U mL−1。最终通过在评估的最佳条件下进行的批量实验验证了所获得的结果，并证明以农工业残留物为底物的SuOC可以用作荧光假单胞菌生产脂肪酶的廉价基础（碳源） （NRLL B-2641）。