OBJECTIVE This study aimed to understand the relationship between tissue factor (TF) and laryngeal carcinoma. METHODS Differences in TF expression between pericarcinomatous and carcinomatous tissues were studied in patients with laryngeal carcinoma; the potential clinical significance of the observed differences is discussed. Immunohistochemical, western blot, and RT-PCR analyses were performed to assess the expression of TF at the protein and mRNA levels, and differences between pericarcinomatous and carcinomatous tissues in patients (n = 20) with laryngeal carcinoma were analyzed. RESULTS Expression of TF was significantly higher in pericarcinomatous tissues than in carcinomatous tissues (P < 0.01); furthermore, the intensity of TF mRNA expression was also significantly stronger in pericarcinomatous than in carcinomatous tissue (P < 0.001). Robust expression of TF was observed in pericarcinomatous tissues but not in carcinomatous tissues. CONCLUSION TF may contribute to the carcinogenesis and development of laryngeal carcinoma and may provide a marker for assessment of the degree of malignancy and the progression of laryngeal carcinoma. TF may also provide a new target for therapeutics for human head and neck cancer.

中文翻译：

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组织因子在喉癌患者的癌旁组织中强烈表达。

目的本研究旨在了解组织因子（TF）与喉癌之间的关系。方法研究喉癌患者癌旁组织和癌旁组织中TF表达的差异。讨论了观察到的差异的潜在临床意义。进行了免疫组织化学，蛋白质印迹和RT-PCR分析，以评估TF在蛋白质和mRNA水平上的表达，并分析了喉癌患者（n = 20）的癌旁和癌旁组织之间的差异。结果癌旁组织中TF的表达明显高于癌旁组织（P <0.01）。此外，癌旁瘤组织中TF mRNA表达的强度也明显高于癌组织（P <0。001）。在癌旁组织中观察到了TF的强表达，而在癌组织中未观察到。结论TF可能有助于喉癌的发生和发展，并可能为评估喉癌的恶性程度和进展提供标志。TF也可能为人类头颈癌的治疗提供新的靶点。