During the 1950s and 1970s the osprey (Pandion haliaetus) experienced a dramatic population crash and remains of conservation concern in several parts of the world. We isolated 37 microsatellite loci and assessed these in ospreys sampled in the UK and Norway (using mouth swabs/feathers). From 26 loci variable in four ospreys, we selected 13, combined these into two multiplex-PCR sets and included a sex-typing marker. Additional markers confirmed sexes. In 17 ospreys, feather-sampled in central Norway, we found 3-10 alleles per locus. The 13 loci are autosomal (heterozygotes were present in both sexes) and observed heterozygosities ranged from 0.24 to 0.94. The combined probability of identity for the 13 loci was 8.0 × 10-12. These microsatellite loci will be useful for genetic monitoring, parentage analysis and population genetic studies of the osprey.

中文翻译：

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一个多路复用微卫星装置，用于鱼骨（Pandion haliaetus）的非侵入性基因分型和性别鉴定。

在1950年代和1970年代，鱼鹰（Pandion haliaetus）经历了急剧的人口崩盘，并且在世界一些地方仍然受到保护。我们分离了37个微卫星基因座，并在英国和挪威的鱼鹰中进行了评估（使用口腔拭子/羽毛）。从四个鱼鹰的26个基因座变量中，我们选择了13个，将它们组合成两个多重PCR组，并包括一个性别分型标记。其他标记物证实了性别。在挪威中部用羽毛取样的17种鱼鹰中，我们发现每个位点有3-10个等位基因。这13个基因座是常染色体基因座（在两个性别中都存在杂合子），并且观察到的杂合度范围为0.24至0.94。13个基因座的同一性的总概率为8.0×10-12。这些微卫星基因座将有助于遗传监测，