Gastric cancer (GC) is a malignant cancer with poor prognosis. This study aims to investigate the roles of homeobox A10 (HOXA10) in GC and the correlations between HOXA10/CD44 expression and GC prognosis. Based on qRT-PCR and Western Blot analyses in 50 pairs of fresh GC samples and adjacent normal samples, it is identified that HOXA10 was significantly up-regulated in GC tissues at mRNA and protein levels. Cell proliferation, migration, and invasion were enhanced in GC cells with overexpressed HOXA10, while inhibited in cells with silenced HOXA10. Through IPA software, HOXA10 was predicted to interact with CD44 via MSN, which was preliminarily confirmed by using Western Blot. Through immunohistochemistry and tissue microarray (N=264), it is found that HOXA10 expression was significantly correlated with tumor size (P=0.011) and CD44 expression (P<0.001), while CD44 expression was significantly correlated with tumor size (P<0.001), depth of tumor invasion (P<0.001), lymph node metastasis (P<0.001), distant metastasis (P=0.001), UICC stage (P<0.001), histological differentiation (P<0.001), and HOXA10 expression (P<0.001). Additionally, the over-all survival and disease-free survival of HOXA10(+)/CD44(+) patients were dramatically decreased in comparison with that of HOXA10(+)/CD44(-), HOXA10(-)/CD44(+), or HOXA10(-)/CD44(-) patients (P<0.001), suggesting that the combinatory expression of HOXA10 and CD44 was correlated with poor GC prognosis. In conclusion, HOXA10 and CD44 might play roles in GC tumorigenesis, metastasis, and invasion. HOXA10(+)/CD44(+) expression might serve as a prognostic biomarker for GC, which needs more studies to validate.

中文翻译：

---

HOXA10的过表达促进胃癌细胞的增殖，而HOXA10（+）/ CD44（+）是胃癌的潜在预后生物标志物。

胃癌（GC）是一种预后较差的恶性肿瘤。本研究旨在探讨同源盒A10（HOXA10）在GC中的作用以及HOXA10 / CD44表达与GC预后之间的相关性。基于qRT-PCR和Western Blot分析，在50对新鲜的GC样品和邻近的正常样品中，发现HOXA10在mRNA和蛋白质水平上在GC组织中显着上调。在过表达HOXA10的GC细胞中，细胞增殖，迁移和侵袭得到增强，而在沉默HOXA10的细胞中则受到抑制。通过IPA软件，可以预测HOXA10通过MSN与CD44相互作用，这已通过Western Blot初步证实。通过免疫组织化学和组织微阵列（N = 264），发现HOXA10表达与肿瘤大小显着相关（P = 0。011）和CD44表达（P <0.001），而CD44表达与肿瘤大小（P <0.001），肿瘤浸润深度（P <0.001），淋巴结转移（P <0.001），远处转移（P = 0.001），UICC分期（P <0.001），组织学分化（P <0.001）和HOXA10表达（P <0.001）。此外，与HOXA10（+）/ CD44（-），HOXA10（-）/ CD44（+）相比，HOXA10（+）/ CD44（+）患者的总生存率和无病生存率显着降低。 ，或HOXA10（-）/ CD44（-）患者（P <0.001），表明HOXA10和CD44的联合表达与GC预后不良相关。总之，HOXA10和CD44可能在GC肿瘤发生，转移和侵袭中起作用。HOXA10（+）/ CD44（+）表达可能是GC的预后生物标志物，