We describe the development of a chemical process based on the CuAAC reaction (click chemistry) to ligate DNA strands and produce an unnatural triazole backbone linkage. The chemical reaction is templated by a complementary DNA splint which accelerates the reaction and provides the required specificity. The resultant 1,4-triazole linkage is read through by DNA and RNA polymerases and is biocompatible in bacterial and human cells. This work has implications for the synthesis of chemically modified genes and other large modified DNA and RNA constructs.

中文翻译：

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模拟生命系统中 DNA 磷酸二酯基团的三唑键

我们描述了基于 CuAAC 反应（点击化学）的化学过程的发展，以连接 DNA 链并产生非天然的三唑主链键。化学反应以互补 DNA 夹板为模板，可加速反应并提供所需的特异性。生成的 1,4-三唑键被 DNA 和 RNA 聚合酶读取，在细菌和人体细胞中具有生物相容性。这项工作对化学修饰基因和其他大型修饰 DNA 和 RNA 结构的合成具有重要意义。