Epidemiological studies have shown that exposure to airborne particulate matter (PM) can be an important risk factor for some common respiratory diseases. While many studies have shown that PM exposures are associated with inflammatory reactions, the role of specific cellular responses in the manifestation of primary hypersensitivities and the progression of respiratory diseases remains unclear. In order to better understand mechanisms by which PM can exert adverse health effects, more robust approaches to support in vitro studies are warranted. In response to this need, a group of accepted toxicology assays was adapted to create an analytical suite for screening and evaluating the effects of important, ubiquitous atmospheric pollutants on two model human lung cell lines (epithelial and immature macrophage). To demonstrate the utility of this suite, responses to intact diesel exhaust particles (DEP) and mass-based equivalent doses of their organic extracts were examined. Results suggest that extracts have the potential to induce greater biological responses than those associated with their colloidal counterpart. Additionally, macrophage cells appear to be more susceptible to the cytotoxic effects of both intact DEP and their organic extract, than epithelial cells tested in parallel. As designed, the suite provided a more robust basis for characterizing toxicity mechanisms than the analysis of any individual assay. Findings suggest that cellular responses to PM are cell line dependent, and show that the collection and preparation of PM and/or their extracts have the potential to impact cellular responses relevant to screening fundamental elements of respiratory toxicity. Copyright 2015 American Association for Aerosol Research

中文翻译：

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适用于比较模型人肺细胞对柴油机尾气颗粒及其提取物的平行毒性反应的毒理学套件

流行病学研究表明，暴露于空气中的颗粒物 (PM) 可能是一些常见呼吸系统疾病的重要危险因素。虽然许多研究表明 PM 暴露与炎症反应有关，但特定细胞反应在原发性超敏反应和呼吸系统疾病进展中的作用仍不清楚。为了更好地了解 PM 对健康产生不利影响的机制，需要更强有力的方法来支持体外研究。为满足这一需求，一组公认的毒理学试验被改编成一个分析套件，用于筛选和评估重要的、无处不在的大气污染物对两种模型人肺细胞系（上皮细胞和未成熟巨噬细胞）的影响。为了证明该套件的实用性，研究了对完整柴油机尾气颗粒 (DEP) 和基于质量的有机提取物等效剂量的响应。结果表明，提取物比与其胶体对应物相关的提取物有可能引起更大的生物反应。此外，与平行测试的上皮细胞相比，巨噬细胞似乎更容易受到完整 DEP 及其有机提取物的细胞毒性作用的影响。按照设计，该套件为表征毒性机制提供了比分析任何单个测定更可靠的基础。研究结果表明，细胞对 PM 的反应是细胞系依赖性的，并表明 PM 和/或其提取物的收集和制备有可能影响与筛选呼吸毒性基本要素相关的细胞反应。版权所有 2015 美国气溶胶研究协会